Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils
Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils
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DOI：
10.1021/jf051922u
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发表时间：
2006-03-08
影响因子：
6.1
通讯作者：
Anackov, G
中科院分区：
文献类型：
--
作者：
Bozin, B;Mimica-Dukic, N;Anackov, G
罗勒的精油，牛至，和红蓟（Thalumvulgaris L.）采用气相色谱-质谱联用技术分析其抗氧化活性和抑菌活性。抗氧化活性评价为自由基清除能力（RSC），以及对脂质过氧化（LP）的影响。通过测定精油对2，2-二苯基-1-苦基肼（DPPH度）和OH度自由基的清除活性来评估RSC。在Fe ~（2+）/抗坏血酸和Fe ~（2+）/H_2O_2诱导体系中，评价精油的活性对LP的影响。精油表现出非常强的RSC，减少DPPH自由基形成（IC 50）的范围从0.17（牛至）到0.39 μ g/mL（罗勒）。提取T.尽管没有一种精油能达到50%的中和作用（IC 50），但普通精油表现出最高的OH自由基清除活性。所有测试的精油强烈抑制LP，诱导的Fe 2 +/抗坏血酸或Fe 2 +/H2 O2。对13种细菌菌株和6种真菌进行了抗菌活性测试。最有效的抗菌活性是由牛至精油表达的，甚至对铜绿假单胞菌和大肠杆菌的多重耐药菌株也是如此。一个显着的抗真菌活性率的所有检查精油也表现出来。
The essential oils of Ocimum basilicum L., Origanum vulgare L., and Thymus vulgaris L. were analyzed by means of gas chromatography-mass spectrometry and assayed for their antioxidant and antimicrobial activities. The antioxidant activity was evaluated as a free radical scavenging capacity (RSC), together with effects on lipid peroxidation (LP). RSC was assessed measuring the scavenging activity of the essential oils on 2,2-diphenyl-1-picrylhydrazil (DPPH degrees) and OH degrees radicals. Effects on LP were evaluated following the activities of essential oils in Fe2+/ascorbate and Fe2+/H2O2 systems of induction. Essential oils exhibited very strong RSCs, reducing the DPPH radical formation (IC50) in the range from 0.17 (oregano) to 0.39 mu g/mL (basil). The essential oil of T. vulgaris exhibited the highest OH radical scavenging activity, although none of the examined essential oils reached 50% of neutralization (IC50). All of the tested essential oils strongly inhibited LP, induced either by Fe2+/ascorbate or by Fe2+/H2O2. The antimicrobial activity was tested against 13 bacterial strains and six fungi. The most effective antibacterial activity was expressed by the essential oil of oregano, even on multiresistant strains of Pseudomonas aeruginosa and Escherichia coli. A significant rate of antifungal activity of all of the examined essential oils was also exhibited.