Transcription Factor Binding to a DNA Zip Code Controls Interchromosomal Clustering at the Nuclear Periphery
Transcription Factor Binding to a DNA Zip Code Controls Interchromosomal Clustering at the Nuclear Periphery
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DOI：
10.1016/j.devcel.2012.03.012
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发表时间：
2012-06-12
影响因子：
11.8
通讯作者：
Brickner, Jason H.
中科院分区：
文献类型：
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作者：
Brickner, Donna Garvey;Ahmed, Sara;Brickner, Jason H.
酵母中的活性基因可以通过顺式作用的“DNA邮政编码”与核孔复合物的相互作用而靶向到核外围。我们发现具有相同邮政编码的基因聚集在一起。这种聚类是特异性的;通过不同的邮政编码定位于核外围的基因对不会聚集在一起。在异位位点插入两个不同的邮政编码（GRS I或GAS III）诱导了具有该邮政编码的内源性基因的聚类。靶向核周边并与核孔相互作用是基因聚类的先决条件，但聚类可以在核质中维持。最后，我们发现Put 3转录因子识别GRS I邮政编码，以介导靶向NPC和染色体间聚类。这些结果表明，邮编介导的基因在核周围的聚类影响酵母基因组的三维排列。
Active genes in yeast can be targeted to the nuclear periphery through interaction of cis-acting "DNA zip codes" with the nuclear pore complex. We find that genes with identical zip codes cluster together. This clustering was specific; pairs of genes that were targeted to the nuclear periphery by different zip codes did not cluster together. Insertion of two different zip codes (GRS I or GAS III) at an ectopic site induced clustering with endogenous genes that have that zip code. Targeting to the nuclear periphery and interaction with the nuclear pore is a prerequisite for gene clustering, but clustering can be maintained in the nucleoplasm. Finally, we find that the Put3 transcription factor recognizes the GRS I zip code to mediate both targeting to the NPC and interchromosomal clustering. These results suggest that zipcode-mediated clustering of genes at the nuclear periphery influences the three-dimensional arrangement of the yeast genome.