Microquantitative analysis of the intra-acinar profiles of glutamate dehydrogenase in rat liver.
Microquantitative analysis of the intra-acinar profiles of glutamate dehydrogenase in rat liver.
复制标题
大鼠肝脏中谷氨酸脱氢酶腺泡内分布的微定量分析。
DOI：
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发表时间：
1991
影响因子：
3.2
通讯作者：
D. Sasse
中科院分区：
文献类型：
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作者：
I. Maly;D. Sasse
在成年雄性和雌性大鼠肝脏中，在50-150 ng的组织样品中微量定量测量NAD（+）和NADP（+）依赖性谷氨酸脱氢酶（GDH）的活性，沿肝窦长度连续沿着显微切割。发现具有NAD+作为辅因子的GDH的总活性比具有NADP+的高约1：2.3的比率。所有腺泡内酶谱，无论性别，GDH活性从门静脉周围开始静脉周围结束的梯度增加。这些发现与GDH在大鼠肝脏中的免疫组织化学定位不同。静脉周围值较高的微量GDH曲线可能表明肝腺泡3区的谷氨酰胺合成更明显。
In adult male and female rat liver, the activity of NAD(+)-and NADP(+)-dependent glutamate dehydrogenase (GDH) was microquantitatively measured in tissue samples of 50-150 ng, microdissected continuously along the sinusoidal length. Total activity of GDH with NAD+ as co-factor was found to be higher by a ratio of about 1:2.3 than with NADP+. All intra-acinar enzyme profiles, irrespective of sex, showed an increasing gradient of GDH activity from the periportal beginning to the perivenous end. These findings are at variance with the immunohistochemical localization of GDH in rat liver. The microquantitative GDH profiles with higher perivenous values could indicate a more pronounced glutamine synthesis in Zone 3 of the liver acinus.
Mg2 对谷氨酸脱氢酶的调节以及 Mg2 对亮氨酸激活的放大作用。
影响因子：
3.6
作者：
Fahien,LA;Teller,JK;Macdonald,MJ;Fahien,CM
通讯作者：
Fahien,CM