Design of photodynamic chitosan hydrogels bearing phthalocyanine-colistin conjugate as an antibacterial agent
Design of photodynamic chitosan hydrogels bearing phthalocyanine-colistin conjugate as an antibacterial agent
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DOI：
10.1016/j.ijbiomac.2019.02.081
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发表时间：
2019-05-15
影响因子：
8.2
通讯作者：
Karimi, Ali Reza
中科院分区：
文献类型：
--
作者：
Bayat, Fahimeh;Karimi, Ali Reza
研制一种新的抗菌系统是治疗多重耐药革兰氏阴性菌(主要是铜绿假单胞菌)引起的伤口的基本医学要求。抗菌光动力疗法(APDT)是治疗细菌感染的一种令人鼓舞的治疗方法。总之，大多数光敏剂的应用都受到其较差的水溶性和破坏革兰氏阴性细菌外膜的效率的限制。在此背景下，我们合成了一种新型的光敏剂--锌酞菁-粘菌素(ZnPc-Col)偶联物，以期改善其与革兰氏阴性菌表面的相互作用。通过在壳聚糖水凝胶中引入ZnPc-Col，克服了酞菁的低溶解性。ZnPc-Col和戊二醛的质量分数对凝胶微观结构的测定有显著影响。采用不同的光谱分析方法、扫描电子显微镜和流变学等测试手段对制备的水凝胶及其性能进行了表征。用1，3-二苯基异苯并呋喃作为单重态氧化学猝灭剂，考察了水凝胶的光敏活性。含锌酞菁-粘菌素加合物的水凝胶与仅含锌酞菁成分的水凝胶相比，APDT效率有所提高，这一增强作用已在铜绿假单胞菌中观察到。(C)《2019年》，爱思唯尔出版。
The fabrication of a new antibacterial system is an essential medical requirement to treat wounds caused by multidrug-resistant Gram-negative bacteria, mostly Pseudomonas aeruginosa. Antibacterial photodynamic therapy (APDT) is an encouraging therapy modality for bacterial infection. Anyhow, the applications of most photo sensitizers are restricted by their poor water solubility and their inefficiency in disruption of the outer membrane of Gram-negative bacteria. In this context, zinc phthalocyanine-colistin (ZnPc-Col) conjugate as a new photosensitizer was synthesized in the hope of improving interaction of phthalocyanine with the surface of Gram-negative bacteria. Overcoming the low solubility of phthalocyanine, was achieved by the incorporation of ZnPc-Col into chitosan hydrogel. The weight percentages of ZnPc-Col and glutaraldehyde had remarkable effects in determining the hydrogel microstructure. Different spectroscopic methods, SEM, and rheological measurements were used to assess the properties of ZnPc-Col and prepared hydrogels. The photosensitizing activity of the hydrogels was investigated by application of 1,3-diphenylisobenzofuran as a singlet oxygen chemical quencher. Hydrogels bearing zinc phthalocyanine-colistin adduct revealed an improved APDT efficiency compared to hydrogels containing just zinc phthalocyanine component This enhancement has been seen against P. aeruginosa. (C) 2019 Published by Elsevier B.V.