Dendritic cells modification during sublingual immunotherapy in children with allergic symptoms to house dust mites
Dendritic cells modification during sublingual immunotherapy in children with allergic symptoms to house dust mites
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DOI：
10.1007/s12519-011-0242-3
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发表时间：
2011-02-01
影响因子：
8.7
通讯作者：
Chini, Loredana
中科院分区：
文献类型：
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作者：
Angelini, Federica;Pacciani, Valentina;Chini, Loredana
树突状细胞（DCs）在Th 2介导的对变应原的炎症反应的起始中的重要性是众所周知的，并且最近已经提出DCs在维持对变应原的耐受性中具有关键作用。本研究的目的是探讨舌下免疫治疗（SLIT）在过敏性哮喘中的成功是否是通过诱导DCs功能的变化介导的。方法：对10例对屋尘螨敏感的过敏性哮喘患儿进行SLIT治疗前后12个月的研究。未成熟DC来源于在白细胞介素（IL）-4和GM-CSF存在下培养6天的外周血单核细胞，并在最后24小时用脂多糖刺激以诱导成熟。SLIT治疗12个月后，来自SLIT治疗患者的成熟DC显示出统计学上显著的CD 86上调缺陷，IL-10增加，结论：SLIT诱导DC功能改变，可能是导致T细胞活化受损或驱动T细胞向调节活性方向发展的原因，从而恢复对变应原的免疫耐受。世界儿科杂志2017;7（1）：24-30
The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions.Methods: Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation.Results: After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production.Conclusion: SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens. World J Pediatr 2017;7(1):24-30