Robust Circadian Rhythms of Gene Expression in Brassica rapa Tissue Culture1[W][OA]
Robust Circadian Rhythms of Gene Expression in Brassica rapa Tissue Culture1[W][OA]
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DOI：
10.1104/pp.110.155465
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发表时间：
2010-04
期刊：
影响因子：
7.4
通讯作者：
Xiaodong Xu;Qiguang Xie;C. R. McClung
中科院分区：
文献类型：
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作者：
Xiaodong Xu;Qiguang Xie;C. R. McClung
生物钟通过使内部生物过程与日常环境周期同步来提供时间上的协调。到目前为止，对植物生物钟的研究主要是以拟南芥为模型，但确定这一模型在其他物种中的适用程度是很重要的。因此，我们对白菜的生物钟功能进行了研究。在拟南芥中，通过时钟调节启动子表达荧光素酶活性的转基因植物中的基因表达分析已被证明是一个有用的工具，尽管与转基因植株再生相关的技术挑战阻碍了这一强大工具在油菜中的实施。生物钟是细胞自主的，节律性已被证明在许多物种的组织培养中持续存在。我们已经建立了一个转基因白菜组织培养系统，可以方便地测量和操纵时钟功能。在白菜外植体诱导的组织培养中，我们证明了几个启动子：LUC报告基因的表达具有昼夜节律。这些节律是温度补偿的，并由光和温度脉冲重置。基因表达的组织培养节律与子叶运动的苗期节律在周期长度上呈显著正相关，表明白菜组织培养的生物钟为植物的生物钟提供了一个很好的模型。
Circadian clocks provide temporal coordination by synchronizing internal biological processes with daily environmental cycles. To date, study of the plant circadian clock has emphasized Arabidopsis (Arabidopsis thaliana) as a model, but it is important to determine the extent to which this model applies in other species. Accordingly, we have investigated circadian clock function in Brassica rapa. In Arabidopsis, analysis of gene expression in transgenic plants in which luciferase activity is expressed from clock-regulated promoters has proven a useful tool, although technical challenges associated with the regeneration of transgenic plants has hindered the implementation of this powerful tool in B. rapa. The circadian clock is cell autonomous, and rhythmicity has been shown to persist in tissue culture from a number of species. We have established a transgenic B. rapa tissue culture system to allow the facile measurement and manipulation of clock function. We demonstrate circadian rhythms in the expression of several promoter:LUC reporters in explant-induced tissue culture of B. rapa. These rhythms are temperature compensated and are reset by light and temperature pulses. We observe a strong positive correlation in period length between the tissue culture rhythm in gene expression and the seedling rhythm in cotyledon movement, indicating that the circadian clock in B. rapa tissue culture provides a good model for the clock in planta.