ESCHERICHIA-COLI DERIVATIVES LACKING BOTH ALCOHOL-DEHYDROGENASE AND PHOSPHOTRANSACETYLASE GROW ANAEROBICALLY BY LACTATE FERMENTATION
ESCHERICHIA-COLI DERIVATIVES LACKING BOTH ALCOHOL-DEHYDROGENASE AND PHOSPHOTRANSACETYLASE GROW ANAEROBICALLY BY LACTATE FERMENTATION
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DOI：
10.1128/jb.171.7.3650-3655.1989
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发表时间：
1989-07-01
影响因子：
3.2
通讯作者：
CLARK, DP
中科院分区：
文献类型：
--
作者：
GUPTA, S;CLARK, DP
缺乏乙醇脱氢酶的大肠杆菌突变体（adh突变体）不能合成发酵产物乙醇，不能在葡萄糖和其他己糖上厌氧生长。同样，磷酸转乙酰酶阴性突变体（pta突变体）既不分泌醋酸盐，也不厌氧生长。然而，当选择携带adh缺失的菌株在葡萄糖上厌氧生长时，分离出自发的pta突变体。携带adh和pta突变的菌株通过体内核磁共振观察，发现其主要发酵产物为乳酸。adh - pta双突变体的各种组合重新获得了在己糖上厌氧生长的能力，相当于同乳酸发酵。与野生型菌株不同，这种adh pta双突变体不能在山梨醇或葡萄糖醛酸上厌氧生长。从氧化还原平衡的角度讨论了影响发酵酶的各种突变菌株的生长特性。
Escherichia coli mutants lacking alcohol dehydrogenase (adh mutants) cannot synthesize the fermentation product ethanol and are unable to grow anaerobically on glucose and other hexoses. Similarly, phosphotransacetylase-negative mutants (pta mutants) neither excrete acetate nor grow anaerobically. However, when a strain carrying an adh deletion was selected for anaerobic growth on glucose, spontaneous pta mutants were isolated. Strains carrying both adh and pta mutations were observed by in vivo nuclear magnetic resonance and shown to produce lactic acid as the major fermentation product. Various combinations of adh pta double mutants regained the ability to grow anaerobically on hexoses, by what amounts to a homolactic fermentation. Unlike wild-type strains, such adh pta double mutants were unable to grow anaerobically on sorbitol or on glucuronic acid. The growth properties of strains carrying various mutations affecting the enzymes of fermentation are discussed in terms of redox balance.