Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples
Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples
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DOI：
10.1007/s00705-022-05602-8
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发表时间：
2022-09-16
影响因子：
2.7
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
--
作者：
Fukase, Yuka;Minami, Fujiko;Nagai, Makoto
哺乳动物正呼肠孤病毒 (MRV) 是一种无包膜双链 RNA 病毒，宿主范围广泛。 MRV在世界范围内普遍存在，在日本，它们已从各种宿主中分离出来，包括人类、狗、猫、野猪和猪，还在污水中发现了它们。然而，日本猪 MRV 尚未得到遗传特征。在研究包括 MRV 在内的猪肠道病毒时，使用 MA104 细胞培养物从日本猪的粪便中分离出 5 种 MRV。 S1基因的遗传分析表明，日本猪MRV分离株可分为MRV-2和MRV-3。全基因组分析表明，日本猪 MRV 表现出遗传多样性，尽管它们与 DDBJ/EMBL/GenBank 数据库中的猪 MRV 序列具有序列相似性。在日本猪、污水和人类的 MRV 毒株中检测到了几种潜在的遗传内重排事件，表明人畜共患传播。此外，在日本猪 MRV 的 M1 和 S1 基因中发现了同源重组事件。这些发现表明日本猪 MRV 的不同品系共享猪 MRV 基因组骨架，并通过遗传内重配和同源重组事件进化。
Mammalian orthoreoviruses (MRVs) are non-enveloped double-stranded RNA viruses with a broad host range. MRVs are prevalent worldwide, and in Japan, they have been isolated from various hosts, including humans, dogs, cats, wild boars, and pigs, and they have also been found in sewage. However, Japanese porcine MRVs have not been genetically characterized. While investigating porcine enteric viruses including MRV, five MRVs were isolated from the feces of Japanese pigs using MA104 cell culture. Genetic analysis of the S1 gene revealed that the Japanese porcine MRV isolates could be classified as MRV-2 and MRV-3. Whole genome analysis showed that Japanese porcine MRVs exhibited genetic diversity, although they shared sequence similarity with porcine MRV sequences in the DDBJ/EMBL/GenBank database. Several potential intragenetic reassortment events were detected among MRV strains from pigs, sewage, and humans in Japan, suggesting zoonotic transmission. Furthermore, homologous recombination events were identified in the M1 and S1 genes of Japanese porcine MRV. These findings imply that different strains of Japanese porcine MRV share a porcine MRV genomic backbone and have evolved through intragenetic reassortment and homologous recombination events.