NGF-evoked sensitization of muscle fascia nociceptors in humans
NGF-evoked sensitization of muscle fascia nociceptors in humans
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DOI：
10.1016/j.pain.2012.04.033
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发表时间：
2012-08-01
期刊：
影响因子：
7.4
通讯作者：
Rukwied, Roman
中科院分区：
文献类型：
--
作者：
Deising, Saskia;Weinkauf, Benjamin;Rukwied, Roman
神经生长因子(NGF)在肌肉注射后会引起几天的局部痛敏，但全身给药后会持续更长时间的肌肉疼痛。由于肌肉筋膜由游离神经末梢密集支配，我们假设NGF对筋膜伤害性感受器具有持久的敏感化作用。在超声引导下，对14名男性志愿者腰椎水平的竖脊肌筋膜注射1mU神经生长因子(溶于100mU L生理盐水)，分别于6h、1、3、7、14、21天进行超敏反应评定。研究机械刺激(恒压和动态冲击)、运动和电刺激引起的竖脊肌收缩引起的疼痛，以及向神经生长因子和生理盐水处理的肌肉筋膜注射100MU L磷酸盐缓冲液(仅在第7天和第14天)时的疼痛。肌肉筋膜内注射不会引起剧烈疼痛。局部热痛阈值在给予NGF和生理盐水(对照)后没有变化。神经生长因子可持续(1-7天)并显著降低压痛、压阈值、运动诱发的肌肉痛和对冲击性刺激的痛敏(12米/S)。注射质子的疼痛在2周内显著升高(P<；0.04)。NGF诱导肌筋膜对机械和化学刺激的敏感化持续2周。由于筋膜中的伤害性感受器似乎特别容易敏化，它们可能导致急性或慢性肌肉疼痛。(C)2012年国际疼痛研究协会。爱思唯尔出版公司版权所有。
Nerve growth factor (NGF) induces local hyperalgesia for a few days after intramuscular injection, but longer-lasting muscle pain upon systemic administration. As the muscle fascia is densely innervated by free nerve endings, we hypothesized a lasting sensitization of fascia nociceptors by NGF. We administered 1 mu g NGF (dissolved in 100 mu L saline) ultrasound-guided to the fascia of the Musculus erector spinae muscle at the lumbar level of 14 male volunteers and assessed hypersensitivity after 6 hours, and 1, 3, 7, 14, and 21 days. Pain upon mechanical stimuli (constant pressure and dynamic impact), upon exercise and electrically induced M. erector spinae contraction, and upon injection of 100 mu L phosphate buffer pH 4 (at day 7 and 14 only) to the fascia of both NGF- and saline-treated muscles, was investigated. Injections into the muscle fascia did not cause acute pain. Local heat pain thresholds were unchanged following NGF and saline (control) administration. NGF evoked a lasting (days 1-7) and significant reduction of pressure pain, pressure thresholds, exercise-evoked muscle pain, and hyperalgesia to impact stimuli (12 m/s). Pain upon injected protons was significantly elevated (P < 0.04) for 2 weeks. NGF induced a sensitization of the muscle fascia to mechanical and chemical stimuli lasting for up to 2 weeks. As nociceptors in the fascia appear to be particularly prone to sensitization, they may contribute to acute or chronic muscle pain. (C) 2012 International Association for the Study of Pain. Published by Elsevier B. V. All rights reserved.