Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.
Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.
复制标题
研究小鼠羊水根据胎儿阶段刺激角质形成细胞分化的能力。
DOI：
10.1016/j.abb.2021.109003
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发表时间：
2021
影响因子：
3.9
通讯作者：
Hitomi K.
中科院分区：
文献类型：
--
作者：
Kuribayashi M;Kawaguchi Y;Teshima H;Yamaguchi H;Tatsukawa H;Hitomi K.
在胎儿发育期间，胎儿皮肤的屏障功能在表皮形成的特定条件下发育。在角质形成细胞分化中，诱导了各种结构蛋白的精心策划的产生和修饰。我们评估了在不同的胎儿阶段的表皮屏障功能，通过评估的酶活性的交联蛋白，转氨酶，和荧光染料的渗透在染色的表皮切片。在妊娠15.5 - 17.5天期间，表皮中的酶活性似乎显著增加;同时，染料渗透显著降低，表明形成了保护屏障。对于早期胎儿表皮形成，羊水中的不明刺激因子可能通过刺激角质形成细胞分化促进屏障功能。因此，我们进行了蛋白质组学光谱（MS）分析的组成部分，在AF在不同的胎儿阶段。此外，我们使用培养的角质形成细胞分化系统研究了组分的促进能力。根据MS分析，AF组分似乎表现出阶段特异性变化，其中可能的独特功能已被确定。我们还发现，将来自每个阶段的AF添加到用于培养的角质形成细胞的培养基中，特异性地增强了分化标记物的水平。这些结果提供了信息的可能作用的AF，含有角质形成细胞分化的调节因子。
During fetal development, the barrier function of the fetal skin is developed under specific conditions for epidermis formation. In keratinocyte differentiation, the well-orchestrated production and modification of various structural proteins are induced. We assessed the epidermal barrier function in different fetal stages by evaluating the enzymatic activity of cross-linking proteins, transglutaminases, and the permeation of fluorescence dye in the stained epidermal sections. During days 15.5–17.5 in gestation, the enzymatic activities in the epidermis appeared to increase significantly; meanwhile, dye permeation was substantially decreased, suggesting the formation of a protective barrier. For the fetal epidermis formation in the earlier stage, unclarified stimulating factors in the amniotic fluid (AF) are possible to promote barrier function by stimulating keratinocyte differentiation. Thus, we performed proteomic spectrometric (MS) analysis on the components in the AF at different fetal stages. Also, we investigated the promotive ability of the components using a cultured keratinocyte differentiation system. According to the MS analysis, the AF components appeared to exhibit stage-specific variations, where possible unique functions have been identified. We also found that adding the AF from each stage to the medium for cultured keratinocytes specifically enhanced the levels of the differentiation markers. These results provide information on the possible role of AF that contains regulatory factors on keratinocyte differentiation.