Normal Values of T, B and NK Lymphocyte Subpopulations in Peripheral Blood of Healthy Cuban Adults
Normal Values of T, B and NK Lymphocyte Subpopulations in Peripheral Blood of Healthy Cuban Adults
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DOI：
10.37757/mr2019.v21.n2-3.5
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发表时间：
2019-04-01
期刊：
影响因子：
2.1
通讯作者：
Mora-Diaz, Isabel
中科院分区：
文献类型：
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作者：
Kokuina, Elena;Breff-Fonseca, Martha C.;Mora-Diaz, Isabel
淋巴细胞亚群的定量可用于评估健康和疾病状态下的免疫应答，包括免疫缺陷、自身免疫、感染和癌症。研究发现，不同细胞亚群的浓度和比例因地理位置、年龄、性别和种族而异。目的探讨正常人外周血淋巴细胞亚群的绝对浓度和百分比及其与性别、年龄的关系。89岁= 50岁。我们纳入了通过书面知情同意同意书同意参与的个人。采用双平台流式细胞仪检测外周血T淋巴细胞亚群CD 3+、CD 3 +/CD 4 +T、CD 3 +/CD 8 +T、CD 19 + B细胞和CD 3-/CD 56+自然杀伤细胞的绝对值和百分比值。我们估计了中位数和2.5和97.5的平均值，并计算了皮尔逊相关系数来评估相关性。显著性检验也用于比较组。结果淋巴细胞绝对值和百分比（%）的范围为：总淋巴细胞：1200-3475个/μ L（20.2-49.3）; CD 3 + T细胞：880-2623个细胞/μ L（56.5-84.7）; CD 3 +/CD 4 + T细胞：479-1792个细胞/μ L（30.3-55.7）; CD 3 +/CD 8 + T细胞：248-1101个细胞/μ L（13.2-42.9）：CD 19 + B细胞：114-1491个细胞/μ L（5.4-49.5）; CD 3-/CD 56+自然杀伤细胞：70-652个细胞/μ L（3.7-28.0）;和CD 4+：CD 8+指数：0.80-3.92。淋巴细胞和CD 3 + T细胞的绝对数量-但不是绝对数量-在那些
INTRODUCTION Quantification of lymphocyte subpopulations is useful for evaluating immune response in states of health and disease, including immunodeficiencies, autoimmunity, infections and cancer. Studies have found that concentrations and proportions of different cell subpopulations vary with geographic location, age, sex and ethnicity. Knowing the normal values of these cells and their variation in healthy populations will contribute to improved clinical practice and scientific research.OBJECTIVE Estimate normal absolute concentrations and percentages of the most abundant lymphocyte subpopulations in peripheral blood and their relation to sex and age.METHODS A cross-sectional analysis was conducted in 129 healthy adults, 61 men and 68 women aged 18-80 years; 89 aged = 50 years. We included individuals who agreed to participate by written informed consent. Exclusion criteria were chronic disease, or use of tobacco, alcohol or medications that can after immune system cell numbers and functions.Through dual platform flow cytometry, we determined absolute and percentage values for T lymphocyte subsets CD3+, CD3+/CD4+T, CD3+/CD8+T, CD19+ B cells and CD3-/CD56+ natural killer cells in peripheral blood, using an 8-color flow cytometer. We estimated medians and the 2.5 and 97.5 percentiles and calculated the pearson correlation coefficient to evaluate associations. Significance tests were also used to compare groups. The significance threshold was p = 0.05 in all cases.RESULTS Ranges of absolute values and percentages (%) were: total lymphocytes: 1200-3475 cells/mu L (20.2-49.3); CD3+ T cells: 880-2623 cells/mu L (56.5-84.7); CD3+/CD4+ T cells: 479-1792 cells/mu L (30.3-55.7); CD3+/CD8+ T cells: 248-1101 cells/mu L (13.2-42.9): CD19+ B cells: 114-1491 cells/mu L (5.4-49.5); CD3-/CD56+ natural killer cells: 70-652 cells/mu L (3.7-28.0); and the CD4+:CD8+ index: 0.80-3.92. Absolute numbers- but not percentages-of lymphocytes and CD3+ T cells were higher in those