Quantity and distribution of eosinophils in the gastrointestinal tract of children
Quantity and distribution of eosinophils in the gastrointestinal tract of children
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DOI：
10.2350/11-05-0130.1
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发表时间：
2006-05-01
影响因子：
1.9
通讯作者：
Rothenberg, Marc E.
中科院分区：
文献类型：
--
作者：
DeBrosse, Charles W.;Case, Justin W.;Rothenberg, Marc E.
关于健康人胃肠道中嗜酸性粒细胞的数量和位置，目前缺乏相关数据。因此，我们检查了在对儿科患者进行内窥镜检查时获得的胃肠道活组织检查。活组织检查以前被解释为没有诊断异常。特应性和非特应性个体的细胞外嗜酸性粒细胞成分和数量被测定。在食道中，仅有2.7%的高倍视野(HPF)出现嗜酸性粒细胞，平均值为0.03+/-0.10 Eos/HPF(平均+/-标准差)，最大值为1/HPF。胃窦和胃底检查发现固有层中的嗜酸性粒细胞数量相似(分别为1.9+/-1.3和2.1+/-2.4个/HPF)，表面上皮内未见嗜酸性粒细胞。在小肠，十二指肠隐窝固有层和回肠固有层分别有9.6+/-5.3(最多26个/HPF)和12.4+/-5.4个/HPF(最多28个/HPF)。表面上皮和隐窝上皮内嗜酸性粒细胞数量最少。在大肠中，盲肠嗜酸性粒细胞密度最高(20.3+/-8.2个/HPF，最大为50个/HPF)，横结肠和乙状结肠较低(分别为16.3+/-5.6和8.3+/-5.9个/HPF)。胃肠道各节段均可见细胞外嗜酸性粒细胞的百分比为70%~93%，活检边缘细胞外颗粒最多(P<；0.05)。特应性和非特应性患者的嗜酸性粒细胞数量相当。这些数据建立了无明显病理疾病的儿科患者的胃肠嗜酸粒细胞基线数值。
There are a lack of data on the quantity and location of eosinophils in the gastrointestinal tract of healthy individuals. Accordingly, we examined gastrointestinal biopsies obtained during endoscopic evaluation of pediatric patients. Biopsies were previously interpreted as having no diagnostic abnormality. The presence of extracellular eosinophil constituents and the quantity of eosinophils in atopic versus nonatopic individuals was determined. In the esophagus, eosinophils were present in only 2.7% of high-power fields (hpf), with a mean value of 0.03 +/- 0.10 eosinophils/hpf (mean +/- standard deviation) and a maximum of 1 eosinophil/hpf. Examination of the antrum and fundus revealed similar numbers of eosinophils in the lamina propria (1.9 +/- 1.3 and 2.1 +/- 2.4 eosinophils/hpf, respectively), with no eosinophils observed in the surface epithelium. In the small intestine, there were 9.6 +/- 5.3 (maximum, 26 eosinophils/hpf) and 12.4 +/- 5.4 eosinophils/hpf (maximum, 28 eosinophils/hpf) in the intercryptal lamina propria of the duodenum and ileum, respectively. The number of eosinophils in the surface epithelium and crypt epithelium was minimal. In the large intestine, the highest concentration of eosinophils was observed in the cecum (20.3 +/- 8.2 eosinophils/hpf, maximum, 50 eosinophils/hpf), and there were lower concentrations in the transverse and sigmoid colon (16.3 +/- 5.6 and 8.3 +/- 5.9 eosinophils/hpf, respectively). The percentage of fields demonstrating extracellular eosinophil granules in all gastrointestinal segments was 70% to 93%, and extracellular granules were most numerous at the edge of the biopsy (P < 0.05). Atopic and nonatopic patients had comparable numbers of eosinophils. These data establish baseline gastrointestinal eosinophil values in pediatric patients without apparent pathological disease.