Efficient Procurement of Epithelial Stem Cells from Human Tissue Specimens Using a Rho-Associated Protein Kinase Inhibitor Y-27632
Efficient Procurement of Epithelial Stem Cells from Human Tissue Specimens Using a Rho-Associated Protein Kinase Inhibitor Y-27632
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DOI：
10.1089/ten.tea.2009.0339
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发表时间：
2010-04-01
影响因子：
4.1
通讯作者：
Vogel, Jonathan C.
中科院分区：
文献类型：
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作者：
Terunuma, Atsushi;Limgala, Renuka Pudi;Vogel, Jonathan C.
皮肤和角膜等上皮组织干细胞的有效培养对于组织工程的实验研究和临床应用都很重要。我们现在证明，在原代培养的最初 6 天，用 Rho 相关蛋白激酶抑制剂 Y-27632 处理人皮肤来源的角质形成细胞，可以增加具有干细胞特性的角质形成细胞的数量，在新鲜分离的细胞的体外培养和随后的传代过程中形成集落（50 倍）。此外，我们表明 Y-27632 治疗可以增加源自人类前列腺样本的前列腺上皮细胞的总数。因此，在原代培养过程中使用Y-27632提供了一种简单有效的方法，可以从各种人类上皮组织中制备大量上皮干细胞。
The efficient culture of stem cells from epithelial tissues such as skin and corneas is important for both experimental studies and clinical applications of tissue engineering. We now demonstrate that treatment of human-skin-derived keratinocytes with a Rho-associated protein kinase inhibitor Y-27632 for the initial 6 days of primary culture can increase the number of keratinocytes that possess stem cell properties to form colonies during in vitro culture of freshly isolated cells and subsequent passage (50-fold). Further, we show that Y-27632 treatment can increase the total number of prostate epithelial cells derived from human prostate specimens. Therefore, the use of Y-27632 during primary cultures offers a simple and effective way to prepare a large number of epithelial stem cells from various human epithelial tissues.