Anti-Candida activity of Quercus infectoria gall extracts against Candida species.
Anti-Candida activity of Quercus infectoria gall extracts against Candida species.
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DOI：
10.4103/0975-7406.148742
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发表时间：
2015-01
影响因子：
0.8
通讯作者：
Abdul Wahab WN
中科院分区：
文献类型：
--
作者：
Baharuddin NS;Abdullah H;Abdul Wahab WN
没食子栎的虫瘿传统上用于治疗常见疾病，包括由念珠菌属引起的酵母感染。本研究旨在评价Q的体外抗念珠菌活性。没食子提取物对选择的念珠菌属物种的抑制作用。Q.测试没食子菌瘿对白色念珠菌、克鲁斯念珠菌、光滑念珠菌、近平滑念珠菌和热带念珠菌的抗念珠菌活性。使用浓度范围为16 mg/ml至0.03 mg/ml的两倍连续稀释技术确定最低抑制浓度。24小时后，通过传代培养威尔斯孔确定最低杀真菌浓度，其在琼脂平板上未显示浑浊。通过植物化学定性试验筛选粗提物中潜在的植物化学基团，随后进行气相色谱-质谱分析。甲醇提取物和水提取物均表现出显著的抗念珠菌活性，两种粗提物的主要成分均为邻苯三酚。研究结果表明，Q.没食子没食子提取物是开发为抗念珠菌病的潜在来源。
Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species. This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species. Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis. The minimum inhibitory concentrations were determined using the two-fold serial dilution technique of concentrations ranging from 16 mg/ml to 0.03 mg/ml. After 24 h, the minimum fungicidal concentrations were determined by subculturing the wells, which showed no turbidity on the agar plate. Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis. Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts. Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.