Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.
Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.
复制标题
鱼视网膜色素上皮 (RPE) 细胞中的黑素体运动。
DOI：
10.1007/978-1-4939-3124-8_17
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
King-Smith,Christina
中科院分区：
文献类型：
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作者：
King-Smith,Christina
已经开发了几种模型系统来研究细胞内细胞器运动的机制和调节。鱼视网膜色素上皮（RPE）细胞代表了一种用于研究细胞器运动的新颖而简单的系统。分离的 RPE 细胞的原代培养物很容易制备并且适合运动性研究。在体内，鱼类 RPE 内含黑色素的色素颗粒（黑素体）响应光通量迁移距离可达 100 μm。当从上皮层解离并在体外培养时，RPE细胞附着在基底上，其顶端突起从中央细胞体径向延伸。黑素体可以通过化学方式触发聚集或分散在整个投影中，并且可以使用相差显微镜轻松观察到。 RPE 顶端投影中的黑素体迁移依赖于肌动蛋白丝，因此使该模型系统可用于研究肌动蛋白依赖性细胞器运动。
Several model systems have been developed to investigate mechanism and regulation of intracellular organelle motility. The fish retinal pigment epithelial (RPE) cell represents a novel yet simple system for the study of organelle motility. Primary cultures of dissociated RPE cells are easily prepared and amenable to motility studies. In vivo, melanin-containing pigment granules (melanosomes) within fish RPE migrate distances up to 100 μm in response to light flux. When dissociated from the epithelial layer and cultured in vitro,RPE cells attach to the substrate with the apical projections extending radially from the central cell body. Melanosomes can be chemically triggered to aggregate or disperse throughout the projections, and are easily observed using phase contrast microscopy. Melanosome migration in RPE apical projections is dependent on actin filaments, and thus renders this model system useful for investigations of actin-dependent organelle motility.