THE PRECONDITIONED HIPPOCAMPUS ACCELERATES HSP70 HEAT-SHOCK GENE-EXPRESSION FOLLOWING TRANSIENT ISCHEMIA IN THE GERBIL
THE PRECONDITIONED HIPPOCAMPUS ACCELERATES HSP70 HEAT-SHOCK GENE-EXPRESSION FOLLOWING TRANSIENT ISCHEMIA IN THE GERBIL
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DOI：
10.1016/0304-3940(93)90661-4
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发表时间：
1993-05-28
影响因子：
2.5
通讯作者：
KOGURE, K
中科院分区：
文献类型：
--
作者：
AOKI, M;ABE, K;KOGURE, K
求值。研究了沙鼠海马缺血耐受机制、热休克蛋白（HSP） 70 mRNA和免疫反应性HSP70蛋白的诱导。单次缺血3.5 min后，所有海马细胞均有HSP70 mRNA的表达。然而，海马CA1细胞仅产生最低限度的HSP70蛋白，并且细胞在7天后几乎消失。预处理2 min后缺血3.5 min， CA1细胞产生强烈的免疫反应性HSP70信号，7 d时大量CA1细胞存活。与单次缺血相比，CA1细胞HSP70 mRNA诱导的峰值时间向再灌注早期转移。这种HSP70表达的加速变化可能对海马CA1神经元获得缺血耐受起重要作用。
To evaluate the.mechanism of tolerance for ischemia, inductions of heat shock protein (HSP) 70 mRNA and immunoreactive HSP70 protein were studied in the preconditioned gerbil hippocampus. Following the single 3.5-min ischemia, HSP70 mRNA was induced in all hippocampal cells. However, the hippocampal CA1 cells produced only a minimum HSP70 protein, and the cells were almost lost by 7 days. Following the 3.5-min ischemia after 2-min pretreatment, the CA1 cells produced a strong immunoreactive HSP70 signal and large populations of the CA1 cells survived at 7 days. The peak time of the HSP70 mRNA induction shifted to earlier period of reperfusion in the CA1 cells as compared to the case with single ischemia. This accelerated change of HSP70 expression could play an important role for the acquisition of ischemic tolerance of the hippocampal CA1 neurons.