3D bioprinting: improving in vitro models of metastasis with heterogeneous tumor microenvironments.
3D bioprinting: improving in vitro models of metastasis with heterogeneous tumor microenvironments.
复制标题
3D生物打印：通过异质肿瘤微环境改善体外转移模型。
DOI：
10.1242/dmm.025049
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发表时间：
2017-01-01
影响因子：
4.3
通讯作者：
Miller JS
中科院分区：
文献类型：
--
作者：
Albritton JL;Miller JS
即使在过去几十年中在治疗方面取得了许多进展，癌症仍然是全球死亡的主要原因。尽管转移和死亡率增加之间的关系是公认的，但令人惊讶的是，对转移进展的确切机制知之甚少。目前可用的体外模型不能充分复制肿瘤微环境的三维性和异质性以概括体内肿瘤的许多已知特征。因此，我们对转移进展的理解将通过体外模型的发展得到加强，这些模型可以更完全地捕获癌症生物学的显著特征。二十多年来，生物工程小组一直致力于创造用于再生医学和组织工程的体外微环境。在这段时间里，3D打印技术和生物材料研究的进步共同导致了3D生物打印的诞生，这提高了我们开发体外模型的能力，其复杂性接近体内肿瘤微环境。在这篇综述中，我们概述了为组织工程开发的3D生物打印方法，这些方法可以直接应用于构建异质性肿瘤微环境的体外模型。我们讨论了与3D打印相关的考虑因素和局限性，并强调了如何利用这些进展来更好地模拟转移，并可能指导抗癌策略的发展。总结：在这里，Albritton和米勒讨论了最近的3D生物打印进展，这些进展可用于产生异质性肿瘤微环境，以改善癌症转移的物理建模。
Even with many advances in treatment over the past decades, cancer still remains a leading cause of death worldwide. Despite the recognized relationship between metastasis and increased mortality rate, surprisingly little is known about the exact mechanism of metastatic progression. Currently available in vitro models cannot replicate the three-dimensionality and heterogeneity of the tumor microenvironment sufficiently to recapitulate many of the known characteristics of tumors in vivo. Our understanding of metastatic progression would thus be boosted by the development of in vitro models that could more completely capture the salient features of cancer biology. Bioengineering groups have been working for over two decades to create in vitro microenvironments for application in regenerative medicine and tissue engineering. Over this time, advances in 3D printing technology and biomaterials research have jointly led to the creation of 3D bioprinting, which has improved our ability to develop in vitro models with complexity approaching that of the in vivo tumor microenvironment. In this Review, we give an overview of 3D bioprinting methods developed for tissue engineering, which can be directly applied to constructing in vitro models of heterogeneous tumor microenvironments. We discuss considerations and limitations associated with 3D printing and highlight how these advances could be harnessed to better model metastasis and potentially guide the development of anti-cancer strategies. Summary: Here, Albritton and Miller discuss recent 3D bioprinting advances that can be used to produce heterogeneous tumor microenvironments for improved physical modeling of cancer metastasis.