Automated RNA selection
Automated RNA selection
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DOI：
10.1021/bp980097h
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发表时间：
1998-11-01
影响因子：
2.9
通讯作者：
Ellington, AD
中科院分区：
文献类型：
--
作者：
Cox, JC;Rudolph, P;Ellington, AD
体外选择可用于产生靶向分子的核酸配体（适体），所述靶向分子的大小和结构范围从阳离子到细胞。然而，选择过程是重复和耗时的。我们已经自动化的协议，在体外选择使用增强比克曼Biomek 2000移液机器人。自动化选择程序需要集成四种设备并优化四种分子生物学方法，是迄今为止尝试的最复杂的自动化方案之一。最初的选择尝试产生了强大的复制寄生虫，但自动化选择程序的优化抑制了这些寄生虫的出现，并导致选择真正的核酸配体。自动选择现在可以用于在几天而不是几周或几个月内生成核酸适体。
In vitro selection can be used to generate nucleic acid ligands (aptamers) to target molecules ranging in size and structure from cations to cells. However, the selection process is repetitive and time-consuming. We have automated a protocol for in vitro selection using an augmented Beekman Biomek 2000 pipetting robot. The automated selection procedure requires the integration of four devices and the optimization of four molecular biology methods, and is one of the most complex automated protocols attempted to date. Initial attempts at selection yielded robust replication parasites, but optimization of the automated selection procedure suppressed the emergence of these parasites and led to the selection of true nucleic acid ligands. Automated selection can now be used to generate nucleic acid aptamers in days rather than weeks or months.