Moxibustion at CV4 alleviates atherosclerotic lesions through activation of the LXRα/ABCA1 pathway in apolipoprotein-E-deficient mice
Moxibustion at CV4 alleviates atherosclerotic lesions through activation of the LXRα/ABCA1 pathway in apolipoprotein-E-deficient mice
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DOI：
10.1136/acupmed-2016-011317
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Zhao, Baixiao
中科院分区：
文献类型：
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作者：
Cui, Yingxue;Liu, Juntian;Zhao, Baixiao
目的：探讨艾灸的抗动脉粥样硬化作用及其是否通过胆固醇逆向转运过程介导。研究方法：8周龄雄性ApoE基因敲除小鼠随机分为动脉粥样硬化组（AS）和AS+艾灸组（AS+M），每组10只。选择相同背景的C57 BL/6 J小鼠（n=10）作为对照。AS+M组采用点燃的艾草在CV 4上方间接灸。将AS组和对照组小鼠束缚在同一保持器中，用未点燃的艾草保持在CV 4上。所有治疗均为20 min/d，6 d/周，共12周。处理后，将小鼠安乐死并测量其血清脂质。采集主动脉根部和胸主动脉，分别进行苏木精-伊红和红油O染色，以分析动脉粥样硬化病变。Western blotting检测胸主动脉三磷酸腺苷结合盒（ABCA）A1/G1和肝X受体α（LXR α）的表达。结果如下：与AS小鼠相比，经黄芪治疗的（AS+M）小鼠显示主动脉根部和胸主动脉中斑块面积百分比显著降低，胸主动脉中LXR α和ABCA 1表达升高。在AS+M组和AS组小鼠之间，胸主动脉中的平均脂质面积百分比或胸主动脉中的ABCG 1表达没有显著差异。结论：莫西沙星CV 4治疗可抑制ApoE（-/-）小鼠动脉粥样硬化病变的进展。艾灸的抗动脉粥样硬化作用可能通过以下方式实现：（1）调节脂质代谢，从而防止脂质积聚;（2）上调病变区域LXR α和ABCA 1介导的胆固醇流出。
Objectives: To investigate the anti-atherogenic effect of moxibustion and whether it is mediated through the reverse cholesterol transport process. Methods: 8-week-old male apolipoprotein E deficient (ApoE(-/-) knockout) mice were randomly divided into two groups (n=10 per group): atherosclerosis (AS) and AS plus moxibustion (AS+M). C57BL/6J mice of the same background (n=10) were selected as controls. Mice in the AS+M group received indirect moxibustion with an ignited moxa stick held over CV4. Mice of the AS and control groups were restrained in the same holder with an unlit moxa stick held over CV4. All treatments were performed for 20 min per day, 6 days per week for 12 weeks. After the treatment, the mice were euthanased and their serum lipids were measured. The aortic roots and thoracic aortas were collected for haematoxylin and eosin and red oil O staining, respectively, to analyse the atherosclerotic lesions. Expression of adenosine triphosphate binding cassette (ABCA)A1/G1 and liver X receptor alpha (LXR alpha) in the thoracic aorta were examined with Western blotting. Results: The moxibustion-treated (AS+M) mice showed a significantly lower plaque area percentage in the aortic root and thoracic aorta, and higher expression of LXR alpha and ABCA1 in the thoracic aorta compared with the AS mice. No significant differences were found in average lipid area percentage in the thoracic aorta, or ABCG1 expression in the thoracic aorta, between mice in the AS+M and AS groups. Conclusion: Moxibustion treatment at CV4 suppressed the progression of atherosclerotic lesions in ApoE(-/-) mice. The anti-atherogenic effect of moxibustion may be achieved by: (1) regulation of lipid metabolism, and thus prevention of lipid accumulation; and (2) upregulation of LXR alpha- and ABCA1-mediated cholesterol efflux in the lesion area.