Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix
Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix
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DOI：
10.1080/03079457.2011.554797
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发表时间：
2011-01-01
期刊：
影响因子：
2.8
通讯作者：
Olah, I.
中科院分区：
文献类型：
--
作者：
Biro, E.;Kocsis, K.;Olah, I.
应用免疫组织化学方法研究了传染性法氏囊病病毒（IBDV）F52/70株感染对脾脏细胞外基质（ECM）的影响。ECM的主要纤维成分，I型和III型胶原蛋白和主要ECM组织糖蛋白（层粘连蛋白，腱生蛋白和纤连蛋白）进行了监测，直到感染后11天（d.p.i.）。到了3 d.p.i.，形成脾的抗原捕获区的基本支架的胶原被破坏，随后是糖蛋白的劣化。红髓和白色髓其他区域（动脉周围淋巴鞘和生发中心）的ECM似乎正常。脾脏两个区域之间ECM中显著不同的病理学改变的原因可以通过网状细胞的起源来解释。在抗原捕获区和其他脾脏区域的网状细胞分别是造血和间充质来源。可能造血来源的网状细胞比间充质细胞更易感染IBDV。脾白色髓的抗原捕获、B细胞依赖区的发育先于动脉周围淋巴鞘和生发中心的发育，这表明该区域可能有助于B细胞成熟。抗原捕获区中ECM的损伤导致组织组织结构受损，这可能有助于永久性免疫抑制。
The effects of infectious bursal disease virus (IBDV) (strain F52/70) infection were studied by immunohistochemical methods on the splenic extracellular matrix (ECM). The major fibrillar components of the ECM, the type I and type III collagens and the main ECM organizing glycoproteins (laminin, tenascin and fibronectin) were monitored up to 11 days post-infection (d.p.i.). By 3 d.p.i., the collagens that form the basic scaffold of the antigen-trapping region of the spleen are destroyed, which is followed by deterioration of the glycoproteins. The ECM in the red pulp and the other regions of the white pulp (periarteriolar lymphatic sheath and germinal centre) seem to be normal. The reason for the significantly different pathological alterations in the ECM between the two regions of the spleen may be explained by the origin of the reticular cells. The reticular cells in the antigen-trapping zone and other splenic regions are of haemopoietic and mesenchymal origins, respectively. Possibly, the reticular cells of the haemopoietic origin are more susceptible for the IBDV infection than the mesenchymal ones. Development of the antigen-trapping, B-cell-dependent zone of the splenic white pulp precedes that of the periarteriolar lymphatic sheath and germinal centre, which suggests that this region may contribute to B-cell maturation. Damage of the ECM in the antigen-trapping zones results in impairment of tissue organization, which may contribute to the permanent immunosuppression.