Iron mediated hematological, oxidative and histological alterations in freshwater fish Labeo rohita
Iron mediated hematological, oxidative and histological alterations in freshwater fish Labeo rohita
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DOI：
10.1016/j.ecoenv.2018.11.129
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发表时间：
2019-04-15
影响因子：
6.8
通讯作者：
Pandey, Pramod Kumar
中科院分区：
文献类型：
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作者：
Singh, Mamta;Barman, Anindya Sundar;Pandey, Pramod Kumar
铁是几种生物体的许多生理功能的必需元素，但过量会导致毒性。山区水体中的高铁含量被认为是导致水产养殖系统生产力低下的主要因素之一。但是，迄今为止，关于铁超载对水生生物，特别是养殖鱼类的不良影响的综合报道很少。因此，本研究进行了调查的不利影响，铁超载的经济重要的水产养殖鱼类品种野唇鱼。铁（亚铁）的三种亚致死试验浓度，即，将1/16、1/8和1/4的LC 50（96 h后），即8.25、16.51和33.01 mg L-1分别用于体内暴露。在24、48、72和96 h的时间间隔对对照和暴露样本的血细胞和组织样本进行采样，以评估血液学、氧化应激和组织学参数的变化。在暴露的鱼中观察到红细胞和白细胞计数、血红蛋白、脂质过氧化、抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）和组织铁积累的显著变化。脂质过氧化的显着增加，再加上自由基清除剂，如超氧化物歧化酶和过氧化氢酶显着减少，揭示了一个妥协的抗氧化防御机制暴露于铁超载的鱼类。鳃和肝脏组织学检查显示严重的组织损伤和组织学改变。发现严重程度以时间和浓度依赖性方式增加。Perl染色显示暴露鱼的肝脏中积累了过量的铁。本研究所观察到的病理生理变化为铁超载应激在L。rohita。
Iron is an essential element for many physiological functions of several organisms but in excess it causes toxicity. High iron content in water bodies of mountainous states is considered as one of the major factor, responsible for low productivity in aquaculture systems. But, till date comprehensive reports on the adverse effect of iron overload in aquatic organisms, especially cultured fishes are scanty. Therefore, the present study was undertaken to investigate the adverse effects of iron overload in economically important aquaculture fish species Labeo rohita. Three sub-lethal test concentration of iron (ferrous) viz., 1/16th, 1/8th and 1/4th of LC50 (post 96 h) i.e. 8.25, 16.51 and 33.01 mg L-1 , respectively, were used for in vivo exposure. Blood cells and tissue samples of the control & exposed specimens were sampled at intervals of 24, 48, 72 and 96 h to assess alterations in hematological, oxidative stress and histological parameters. Significant changes in erythrocyte and leukocyte counts, hemoglobin, lipid peroxidation, antioxidant enzyme activity (super oxide dismutase and catalase) and tissue iron accumulation were observed in the exposed fish. Significant increase in lipid peroxidation, coupled with significant reduction in free radicals scavengers like super oxide dismutase and catalase revealed a compromised anti-oxidative defense mechanism in the fishes exposed to iron overload. Histological examination of gills and liver showed severe tissue injury and histological alternations. Severity was found to increase in time and concentration dependent manner. Perl's staining revealed accumulation of excess iron in liver of the exposed fish. The observed patho-physiological changes in the present study provide the most comprehensive insight of iron overload stress in L. rohita.