Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines.
Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines.
复制标题
对肺支原体温度敏感突变体作为可能的啮齿动物疫苗的生物学评价。
DOI：
10.1128/iai.58.7.2289-2296.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Pakes,SP
中科院分区：
文献类型：
--
作者：
Lai,WC;Bennett,M;Lu,YS;Pakes,SP
通过用N-甲基-N '-硝基-N-亚硝基胍处理肺支原体野生型菌株产生温度敏感突变株（TSM）。选择38 ℃作为限制温度和34 ℃作为允许温度的三种TSM。两种TSM，UTCMI和UTCMII，被证明是非致病性的，但免疫原性。此外，它们不会诱发肺炎、气管炎或中耳炎，但会诱发轻度鼻炎。经体外和体内传代10代后，其稳定性良好。它们在接种疫苗的大鼠中引起了良好的抗体产生和细胞介导的免疫。它们对大鼠淋巴细胞也没有促有丝分裂作用。用这些TSM鼻内免疫的大鼠显着保护免受野生型生物体的攻击。这些突变体在形态学和血清学上与野生型微生物无法区分。生长特性和抗生素敏感性与野生型生物相似，只是它们只能在34 ℃下生长。与野生型微生物相反，它们不结合或裂解绵羊红细胞。因此，这些TSM可作为预防M的疫苗。大鼠肺感染。
Temperature-sensitive mutants (TSMs) of Mycoplasma pulmonis were produced by treating the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine. Three TSMs were selected at 38 degrees C, as a restrictive temperature, and at 34 degrees C, as a permissive temperature. Two TSMs, UTCMI and UTCMII, were proven to be nonpathogenic but immunogenic. In addition, they did not induce pneumonia, tracheitis, or tympanitis but did induce mild rhinitis. They were stable after 10 passages in vitro and in vivo. They elicited excellent antibody production and cell-mediated immunity in vaccinated rats. They also were not mitogenic to rat lymphocytes. Rats immunized intranasally with these TSMs were significantly protected against challenge with wild-type organisms. These mutants were morphologically and serologically indistinguishable from the wild-type organisms. The growth characteristics and antibiotic sensitivities were similar to those of wild-type organisms, except that they grew only at 34 degrees C. In contrast to wild-type organisms, they did not bind to or lyse sheep erythrocytes. Thus, these TSMs may qualify as a vaccine to prevent M. pulmonis infection in rats.