Proteome analysis of programmed cell death and defense signaling using the rice lesion mimic mutant cdr2
Proteome analysis of programmed cell death and defense signaling using the rice lesion mimic mutant cdr2
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DOI：
10.1094/mpmi-18-0052
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发表时间：
2005-01-01
影响因子：
3.5
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Tsunezuka, H;Fujiwara, M;Shimamoto, K
我们先前已经在水稻中鉴定了三个类似病斑的突变体，细胞死亡和抗性（cdr）。这些突变体诱导了一系列的防御反应，包括防御相关基因的表达和植物抗毒素的高积累，表明cdr突变体是研究水稻细胞程序性死亡和防御信号的有用材料。在这里，我们进行了cdr2突变体的蛋白质组分析。在损伤形成的三个不同阶段从野生型和cdr 2突变体制备的总蛋白质使用双向电泳进行比较。我们共发现37个蛋白质在cdr 2和野生型之间有差异表达。其中，cdr2突变体中28个点表达上调，9个点表达下调。通过质谱分析鉴定了所有蛋白质点。这些差异调节的蛋白质包括防御相关蛋白。此外，27种蛋白质被归类为代谢酶，这表明在cdr2突变体中发生的程序性细胞死亡与活跃的代谢变化有关。我们的研究表明，蛋白质组分析是一个有用的方法来研究植物细胞程序性死亡和防御信号。
We have previously identified three lesion-mimic mutants, cell death and resistance (cdr), in rice. These mutants induce a series of defense responses, including expression of defense-related genes and high accumulation of phytoalexins, indicating that the cdr mutants are useful materials to study programmed cell death and defense signaling in rice. Here, we carried out a proteome analysis of the cdr2 mutant. Total proteins prepared from the wild type and the cdr2 mutant at three different stages of lesion formation were compared using two-dimensional electrophoresis. We found a total of 37 proteins that were differentially expressed between cdr2 and wild type. Among them, 28 spots were up-regulated and nine were down-regulated in the cdr2 mutant. All the protein spots were identified by mass spectrometric analysis. These differentially regulated proteins included defense-related proteins. In addition, 27 proteins were classified as metabolic enzymes, suggesting that the programmed cell death that occurs in the cdr2 mutant is associated with active metabolic changes. Our study shows that proteome analysis is a useful approach to study programmed cell death and defense signaling in plants.