Characterization of the major apolipoproteins secreted by two human hepatoma cell lines.
Characterization of the major apolipoproteins secreted by two human hepatoma cell lines.
复制标题
两种人肝癌细胞系分泌的主要载脂蛋白的表征。
DOI：
10.1021/bi00528a006
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Knowles,BB
中科院分区：
文献类型：
--
作者：
Zannis,VI;Breslow,JL;SanGiacomo,TR;Aden,DP;Knowles,BB
V. I. Zannis，* J. L. Breslow，T. R. SanGiacomo，D. P.亚丁和B. B。Knowles摘要：两种新描述的人肝癌衍生细胞系Hep G2和Hep 3B [Knowles，BB，Howe，CC，&亚丁，D. P.（1980）Science（华盛顿，DC）209，497-499]，合成并向培养基中分泌大多数主要血浆脱辅基蛋白（apoA-I、apoA-II、apoB、apoC-II、apoC-III和apoE）。合成的载脂蛋白通过培养基的直接二维凝胶分析或通过超离心或免疫沉淀纯化载脂蛋白后的二维分析来鉴定。我们发现，两种肝癌细胞系合成的apoA-I均由两种同功蛋白组成，命名为2和3，它们比主要的apoA-I碱性更强。
V. I. Zannis,* J. L. Breslow, T. R. SanGiacomo, D. P. Aden, andB. B. Knowles abstract: Two newly described human hepatoma derived cell lines, Hep G2 and Hep 3B [Knowles, BB, Howe, CC, & Aden, D. P.(1980) Science (Washington, DC) 209, 497-499], synthesize and secrete into the culture medium most of the major plasma apoproteins (apoA-I, apoA-II, apoB, apoC-II, apoC-III, and apoE). The synthesized apoproteins were identified by direct two-dimensional gel analysis of the culture medium or by two-dimensional analysis following purification of the apoproteins by ultracentrifugation or immunoprecipitation. We found that the apoA-I synthesized by both of the hepatoma cell lines consists of two isoproteins designated 2 and 3 which are more basic than themajor