SNAC4 and SNAC9 transcription factors show contrasting effects on tomato carotenoids biosynthesis and softening
SNAC4 and SNAC9 transcription factors show contrasting effects on tomato carotenoids biosynthesis and softening
复制标题
SNAC4 和 SNAC9 转录因子对番茄类胡萝卜素生物合成和软化表现出不同的影响
DOI：
10.1016/j.postharvbio.2018.05.008
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发表时间：
2018-10-01
影响因子：
7
通讯作者：
Xue, Zhaohui
中科院分区：
文献类型：
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作者：
Kou, Xiaohong;Zhao, Yanan;Xue, Zhaohui
番茄成熟过程中，由于番茄红素的积累和叶绿素和叶绿素浓度的下降而引起的颜色变化。利用病毒诱导基因沉默技术(VIGS)研究了转录因子SNAC4(SINAC48，注册号：NM 001,279,348.2)和SNAC9(SINAC19，注册号：XM 004,236,996.2)在果实成熟过程中的作用。抑制SNAC导致成熟延迟，与乙烯产生减少、番茄红素积累和黄色或橙色果实有关。参与类胡萝卜素途径通量和乙烯生物合成的基因持续下调。沉默SNAC4/9改变了番茄的成熟过程。在SNAC4沉默的果实中，软化相关因子的表达下调，ABA(脱落酸)的含量低于阴性对照，而SNAC9沉默的果实表现出相反的表型。外源ABA促进了SNAC4沉默果实的软化，而去甲二氢愈创木酸(NDGA)则减缓了SNAC9沉默果实的软化。我们的结果表明，依赖于乙烯和ABA的通路分别受SNAC4和SNAC9的调控。
Color change due to lycopene accumulation and decreasing mu-xanthophyll and chlorophyll concentrations occurs during ripening of tomatoes (Solanum lycopersicum). The functions of the transcription factors SNAC4 (SINAC48, Accession number: NM 001,279,348.2) and SNAC9 (SINAC19, Accession number: XM 004,236,996.2) during ripening have been studied using virus-induced gene silencing (VIGS) to obtain SNACs-silenced fruit. Silencing of SNACs led to delayed ripening associated with reduced ethylene production, lycopene accumulation, and yellow or orange fruit. Genes involved in carotenoid pathway flux and ethylene biosynthesis were consistently downregulated. Silencing of SNAC4/9 altered tomato ripening processing. In SNAC4-silenced fruit, softening-related factors were down-regulated and there was less ABA (abscisic acid) than the negative control, whereas SNAC9-slilenced fruit showed an opposite phenotype. Exogenous ABA accelerated softening of SNAC4-silenced fruit, while exogenous nordihydroguaiaretic acid (NDGA) slowed down the softening of SNAC9-silenced fruit. Our results suggest that pathways dependent on ethylene and ABA are regulated by SNAC4 and SNAC9, respectively.