Complete genome sequencing of Peyer's patches-derived Lactobacillus taiwanensis CLG01, a potential probiotic with antibacterial and immunomodulatory activity.
Complete genome sequencing of Peyer's patches-derived Lactobacillus taiwanensis CLG01, a potential probiotic with antibacterial and immunomodulatory activity.
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对源自派尔氏斑的台湾乳杆菌 CLG01（一种具有抗菌和免疫调节活性的潜在益生菌）进行完整基因组测序
DOI：
10.1186/s12866-021-02127-z
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发表时间：
2021-02-27
期刊：
影响因子：
4.2
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Li XY;Li LX;Li Y;Zhou RC;Li B;Gu X;Fu SC;Jin BY;Zuo XL;Li YQ
背景
乳酸菌属是人类和动物胃肠道的重要组成部分，通常被认为是益生菌。台湾乳杆菌长期以来一直被认为是一种益生菌，但对该物种的了解仍处于起步阶段。台湾乳杆菌的基因组信息相当有限。需要对其有益特征进行广泛的表征。
结果
从小鼠派尔氏淋巴集结中分离出台湾乳杆菌新菌株 CLG01。我们通过体外实验建立了其益生菌特征。还对该菌株的完整基因组进行了测序和分析。 L. taiwanensis CLG01 对酸具有很强的耐受性，对胆盐具有一定程度的耐受性，对多种病原菌具有良好的抗菌活性。用热灭活的细菌和台湾乳杆菌 CLG01 的细菌上清液体外处理小鼠 RAW 264.7 巨噬细胞，结果增强了免疫反应并上调了 TNF-α 和 IL-6 的表达。当与脂多糖（LPS）共同处理时，菌株 CLG01 还增加了巨噬细胞的 IL-10 产量。台湾乳杆菌CLG01的完整基因组包含一条1.89 Mb的染色体和两个质粒。进一步的基因组分析揭示了与其对不同应激的抵抗力以及上述有益作用相关的基因的存在。此外，在台湾乳杆菌CLG01的基因组中还发现了编码抗菌肽的生物合成基因簇（BGC），如细菌素、线性含唑（in）e肽（LAP）和羊毛硫肽。
结论
台湾乳杆菌 CLG01 是从小鼠派尔氏淋巴结中分离得到的，是第一个对表型和基因型进行系统研究的台湾乳杆菌菌株。这些初步数据证实了台湾乳杆菌 CLG01 作为具有抗菌和免疫调节活性的潜在益生菌候选者的作用，这为进一步研究该物种提供了见解。
Background
The genus Lactobacillus is an important component of the gastrointestinal tract of human and animals and commonly considered as probiotic. L. taiwanensis has long been proposed to be a probiotic whereas understanding on this species is still in its infancy. Genomic information of L. taiwanensis is fairly limited. Extensive characterization of its beneficial traits is needed.
Results
A new strain CLG01 of L. taiwanensis was isolated from mouse Peyer’s patches. We established its probiotic profile through in vitro experiments. Complete genome of this strain was also sequenced and analyzed. L. taiwanensis CLG01 showed robust tolerance to acid and a degree of tolerance to bile salt with a promising antibacterial activity against a broad spectrum of pathogenic bacteria. In vitro treatment of mouse RAW 264.7 macrophage cells with heat-killed bacteria and bacterial supernatant of L. taiwanensis CLG01 resulted in enhancement of immune responses and upregulated expression of TNF-α and IL-6. The strain CLG01 also increased the IL-10 production of macrophages when co-treated with lipopolysaccharide (LPS). Complete genome of L. taiwanensis CLG01 contained a 1.89 Mb chromosome and two plasmids. Further genomic analysis revealed the presence of genes related to its resistance to different stresses and the beneficial effects mentioned above. Moreover, biosynthetic gene clusters (BGCs) encoding antimicrobial peptides, like bacteriocin, linear azol(in)e-containing peptide (LAP) and lanthipeptide, were also identified in the genome of L. taiwanensis CLG01.
Conclusions
L. taiwanensis CLG01, isolated from mouse Peyer’s patches, is the first L. taiwanensis strain with both phenotypes and genotypes systematically studied. These preliminary data confirmed the role of L. taiwanensis CLG01 as a potential probiotic candidate with antibacterial and immunomodulatory activity, which provide insight for further investigation to this species.
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