Adult T-cell leukemia-derived factor/thioredoxin, produced by both human T-lymphotropic virus type I- and Epstein-Barr virus-transformed lymphocytes, acts as an autocrine growth factor and synergizes with interleukin 1 and interleukin 2.
Adult T-cell leukemia-derived factor/thioredoxin, produced by both human T-lymphotropic virus type I- and Epstein-Barr virus-transformed lymphocytes, acts as an autocrine growth factor and synergizes with interleukin 1 and interleukin 2.
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DOI：
10.1073/pnas.87.21.8282
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发表时间：
1990-11
影响因子：
11.1
通讯作者：
Naomi Wakasugi;Yutaka TAGAYAt;Hiro Wakasugi;Akira Mitsui;Michiyuki Maeda;Junji YODOIt;Thomas Tursz-Thom
中科院分区：
文献类型：
--
作者：
Naomi Wakasugi;Yutaka TAGAYAt;Hiro Wakasugi;Akira Mitsui;Michiyuki Maeda;Junji YODOIt;Thomas Tursz-Thom
白细胞介素1（IL-1）已从EB病毒感染的B淋巴母细胞样细胞系3B 6中获得，并显示参与3B 6 B细胞的自分泌生长。成人T细胞白血病衍生因子（ADF）是从人T淋巴细胞病毒I感染的白血病T细胞系（ATL-2）中分离纯化的，并被报道为白细胞介素2（IL-2）受体诱导因子。我们以前报道过3B 6衍生的IL-1和ADF具有相同的分子量、pI和NH 2-末端氨基酸序列。ADF的cDNA克隆与原核二硫键还原酶硫氧还蛋白具有高度的同源性。我们在这里表明，ADF和3B 6衍生的IL-1是相同的。通过RNA印迹，3B 6和ATL-2细胞显示含有高水平的对应于ADF的0.6-脱氢酶mRNA。这种信息在静息的外周血淋巴细胞中未检测到，但可被淋巴细胞活化弱诱导。已经产生了针对对应于ADF的NH 2末端和COOH末端的合成肽的抗体。免疫印迹和顺序免疫沉淀与这些抗体显示相同的13 kDa的蛋白质在3B 6和ATL-2细胞。重组ADF可以维持生长的3B 6和ATL-2细胞在低细胞浓度，没有胎牛血清; ADF，因此，似乎参与他们的自分泌生长。类似地，重组ADF可以增强其他B细胞系的生长，包括EB病毒阴性伯基特淋巴瘤系BL 41和淋巴母细胞样细胞系CRAG 8、CRB 95和1G 8。最后，重组ADF表现出与其他细胞因子如IL-1和IL-2的显著协同作用，使病毒感染的淋巴细胞对各种生长因子的次优量作出反应。
Interleukin 1 (IL-1) has been obtained from the Epstein-Barr virus-infected B-lymphoblastoid cell line 3B6 and shown to be involved in autocrine growth of 3B6 B cells. Independently, adult T-cell leukemia-derived factor (ADF) was purified from human T-lymphotropic virus I-infected leukemic T-cell line (ATL-2) and reported as an interleukin 2 (IL-2) receptor-inducing factor. We have previously reported the same molecular mass, pI, and NH2-terminal amino acid sequence for both 3B6-derived IL-1 and ADF. cDNA cloning of ADF demonstrated high homology with the prokaryotic disulfide reducing enzyme thioredoxin. We show here that ADF and 3B6-derived IL-1 are identical. By RNA blot, 3B6 and ATL-2 cells were shown to contain high levels of 0.6-kilobase mRNA corresponding to ADF. Such message was not detected in resting peripheral blood lymphocytes but could be weakly induced by lymphocyte activation. Antibodies have been raised against synthetic peptides corresponding to the NH2 terminus and the COOH terminus of ADF. Immunoblotting and sequential immunoprecipitation with these antibodies revealed the same 13-kDa protein in 3B6 and ATL-2 cells. Recombinant ADF could sustain growth of 3B6 and ATL-2 cells at low cellular concentration without fetal calf serum; ADF, thus, appears involved in their autocrine growth. Similarly, recombinant ADF could enhance growth of other B-cell lines, including the Epstein-Barr virus-negative Burkitt lymphoma line BL41 and the lymphoblastoid cell lines CRAG8, CRB95, and 1G8. Finally, recombinant ADF exhibits marked synergism with other cytokines, such as IL-1 and IL-2, allowing virally infected lymphocytes to respond to suboptimal amounts of a variety of growth factors.