Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
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DOI：
10.1042/bj20061609
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Brand, Martin D.
中科院分区：
文献类型：
--
作者：
Amo, Taku;Brand, Martin D.
我们介绍了对线粒体DNA（线粒体DNA）变体的生物能量重要性的一般测试：对具有恒定核DNA但不同线粒体DNA的胞质杂交细胞的线粒体中的氧化磷酸化进行模块动力学分析。我们已经将该检验应用于假设[Ruiz-Pesini，Mishmar，布兰登，Procaccio和Wallace（2004）Science 303，223-226]，即在现代人类辐射到较冷气候期间，导致偶联效率降低，导致产生更少ATP和更多热量的特定mtDNA单倍型群（多态性的特定组合）被积极选择。相反，这一假设的预测，从北极单倍群线粒体有类似的，甚至更大的耦合效率比线粒体从热带单倍群。
We introduce a general test of the bioenergetic importance of mtDNA (mitochondrial DNA) variants: modular kinetic analysis of oxidative phosphorylation in mitochondria from cybrid cells with constant nuclear DNA but different mtDNA. We have applied this test to the hypothesis [Ruiz-Pesini, Mishmar, Brandon, Procaccio and Wallace (2004) Science 303, 223-226] that particular mtDNA haplogroups (specific combinations of polymorphisms) that cause lowered coupling efficiency, leading to generation of less ATP and more heat, were positively selected during radiations of modern humans into colder climates. Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups.