Cow-baited tents are highly effective in sampling diverse Anopheles malaria vectors in Cambodia
Cow-baited tents are highly effective in sampling diverse Anopheles malaria vectors in Cambodia
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DOI：
10.1186/s12936-016-1488-y
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发表时间：
2016-08-30
期刊：
影响因子：
3
通讯作者：
Fairhurst, Rick M.
中科院分区：
文献类型：
--
作者：
St Laurent, Brandyce;Oy, Kolthida;Fairhurst, Rick M.
背景：疟疾病媒的准确监测和评价需要有效的采样。本研究的目的是比较柬埔寨户外叮咬按蚊的采样方法。方法：在柬埔寨Pursat省、perah Vihear省和Ratanakiri省，在疟疾传播高峰期连续4夜或6夜对6种不同的捕蚊方法进行评价：人登陆法（HLC）、人诱帐篷法（HBT）、牛诱帐篷法（CBT）、CDC微型灯诱法（LT）、CDC糖蜜酵母微型灯诱法（LT- m）和屏障围栏法(F)。结果：在Pursat、Preah Vihear和Ratanakiri 3个地区共捕获按蚊507只、1175只和615只。cbt每晚捕获的按蚊数量是其他五种采样方法的10到20倍。对2297只按蚊进行形态鉴定和分子分型，分别采用标准形态键和rDNA ITS2区测序区分隐种。总的来说，对27种已知按蚊进行了采样。cbt捕获了与hlc和其他四种陷阱相同的分子物种，以及其他物种。5种按蚊（高压按蚊、严格感barbirostris按蚊、barbirostris III枝按蚊、nivipes按蚊和pedaeniius按蚊）共9个标本感染了恶性疟原虫，并在ct中被捕获。结论：牛诱帐篷对柬埔寨境内多种疟蚊媒介取样效果良好。这种采样方法以有限的采样努力捕获了大量的按蚊，与黄金标准的人类着陆收集相比，大大减少了人类对蚊子叮咬的暴露。
Background: The accurate monitoring and evaluation of malaria vectors requires efficient sampling. The objective of this study was to compare methods for sampling outdoor-biting Anopheles mosquitoes in Cambodia.Methods: In the Cambodian provinces of Pursat, Preah Vihear, and Ratanakiri, six different mosquito trapping methods were evaluated: human landing collection (HLC), human-baited tent (HBT), cow-baited tent (CBT), CDC miniature light trap (LT), CDC miniature light trap baited with molasses and yeast (LT-M), and barrier fence (F) in a Latin square design during four or six consecutive nights at the height of the malaria transmission season.Results: Using all traps, a total of 507, 1175, and 615 anophelines were collected in Pursat, Preah Vihear, and Ratanakiri, respectively. CBTs captured 10- to 20-fold more anophelines per night than the other five sampling methods. All 2297 Anopheles mosquitoes were morphologically identified and molecularly typed using standard morphological keys and sequencing the rDNA ITS2 region to distinguish cryptic species, respectively. Overall, an extremely diverse set of 27 known Anopheles species was sampled. CBTs captured the same molecular species that HLCs and the other four traps did, as well as additional species. Nine specimens representing five Anopheles species (Anopheles hyrcanus, Anopheles barbirostris sensu stricto, Anopheles barbirostris clade III, Anopheles nivipes, and Anopheles peditaeniatus) were infected with Plasmodium falciparum and were exclusively captured in CBTs.Conclusions: These data indicate that cow-baited tents are highly effective in sampling diverse Anopheles malaria vectors in Cambodia. This sampling method captured high numbers of anophelines with limited sampling effort and greatly reduced human exposure to mosquito bites compared to the gold-standard human landing collection.