The effect of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3
The effect of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3
复制标题
DOI：
10.1128/aem.62.10.3594-3599.1996
复制
发表时间：
1996-10-01
影响因子：
4.4
通讯作者：
Dobson, ADW
中科院分区：
文献类型：
--
作者：
OConnor, K;Duetz, W;Dobson, ADW
在恶臭假单胞菌CA-3中的苯乙烯降解以前已被证明在分批培养中受到分解代谢物阻遏。我们在此报告琥珀酸和谷氨酸的分解产物抑制作用，以及有限的无机营养浓度对低生长速率（0.05 h（-1））恒化培养物中恶臭假单胞菌CA-3苯乙烯降解途径的影响。苯乙烯的氧化和氧化苯乙烯异构酶和苯乙醛脱氢酶活性的存在被用作苯乙烯降解途径的表达的量度。在碳源和能量限制的生长条件下，谷氨酸盐和琥珀酸盐均不能抑制菌株降解苯乙烯的能力。较低水平的酶活性的苯乙烯降解途径被认为是在苯乙烯或苯乙酸（PAA）的氨和硫酸盐限制的条件下生长的细胞比在碳和能量的限制。在PAA上生长的细胞在连续培养下氧化苯乙烯和氧化苯乙烯，并具有氧化苯乙烯异构酶和NAD（+）依赖性苯乙醛脱氢酶活性。在硫酸盐限制下，在生长饱和（非限制性）浓度的琥珀酸盐或谷氨酸盐存在下，在苯乙烯或PAA上生长的细胞中观察到苯乙烯代谢的分解代谢物抑制。
Styrene degradation in Pseudomonas putida CA-3 has previously been shown to be subject to catabolite repression in batch culture. We report here on the catabolite-repressing effects of succinate and glutamate and the effects of a limiting inorganic-nutrient concentration on the styrene degradation pathway of P. putida CA-3 in a chemostat culture at low growth rates (0.05 h(-1)). Oxidation of styrene and the presence of styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities were used as a measure of the expression of the styrene degradation pathway. Both glutamate and succinate failed to repress the styrene degradation ability under growth conditions of carbon and energy limitation. Lower levels of enzyme activities of the styrene degradation pathway were seen in cells grown on styrene or phenylacetic acid (PAA) under conditions of both ammonia and sulfate limitation than were seen under carbon and energy limitation. Cells grown on PAA under continuous culture oxidize styrene and styrene oxide and possess styrene oxide isomerase and NAD(+)-dependent phenylacetaldehyde dehydrogenase activities. Catabolite repression of styrene metabolism was observed in cells grown on styrene or PAA in the presence of growth-saturating (nonlimiting) concentrations of succinate or glutamate under sulfate limitation.