Effects of high K+ concentrations on the growth and development of ciliary ganglion neurons in cell culture.
Effects of high K+ concentrations on the growth and development of ciliary ganglion neurons in cell culture.
复制标题
高钾浓度对细胞培养中睫状神经节神经元生长和发育的影响。
DOI：
10.1016/0012-1606(81)90153-6
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Berg,DK
中科院分区：
文献类型：
--
作者：
Nishi,R;Berg,DK
从胚胎眼组织中提取的提取物允许胚胎睫状神经节中存在的所有神经元在细胞培养中存活和发育。高K+浓度刺激培养神经元的生长，高于单独用眼提取物获得的最大水平。在25 mMK+条件下的生长产生了胆碱乙酰转移酶活性、乳酸脱氢酶活性（一种常见的细胞质酶）和每个神经元总蛋白质合成水平的平行增加。K+效应似乎是由膜去极化介导的。细胞内记录证实，在25 mMK+时，神经元慢性去极化。缬草碱对生长产生同样的刺激作用，而河豚毒素阻断了缬草碱的作用，但没有阻止K+的作用。Ca2+也可能在K+效应中发挥作用。两种被认为阻断Ca2+通道的药物（Mg2+和D600）分别阻断或减少了25 mMK+引起的生长增加。在对照培养中，药物不干扰神经元生长，表明眼提取物和膜去极化通过不同的机制影响神经元生长。
Extracts prepared from embryonic eye tissue permit all of the neurons present in embryonic ciliary ganglia to survive and develop in cell culture. High K+concentrations stimulate growth of the neurons in culture above the maximal levels obtained with eye extract alone. Growth in 25 mMK+produced parallel increases in the levels of choline acetyltransferase activity, lactate dehydrogenase activity (a common cytoplasmic enzyme), and total protein synthesis per neuron. The K+effect appears to be mediated by membrane depolarization. Intracellular recording confirmed that the neurons were chronically depolarized in 25 mMK+. Veratridine produced the same stimulation of growth, while tetrodotoxin blocked the veratridine effect without preventing the K+effect. Ca2+may also play a role in the K+effect. Two drugs thought to block Ca2+channels (Mg2+and D600) each blocked or reduced in the increase in growth caused by 25 mMK+. The drugs did not interfere with neuronal growth in control cultures, indicating that eye extract and membrane depolarization influence neuronal growth by different mechanisms.