Impact of hemoglobin breakdown products in the spectral analysis of burn wounds using spatial frequency domain spectroscopy.
Impact of hemoglobin breakdown products in the spectral analysis of burn wounds using spatial frequency domain spectroscopy.
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使用空间频域光谱对烧伤创面进行光谱分析时血红蛋白分解产物的影响。
DOI：
10.1117/1.jbo.24.2.020501
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发表时间：
2019
影响因子：
3.5
通讯作者：
Durkin,AnthonyJ
中科院分区：
文献类型：
--
作者：
Saager,RolfB;Rowland,RebeccaA;Baldado,MelissaL;Kennedy,GordonT;Bernal,NicoleP;Ponticorvo,Adrien;Christy,RobertJ;Durkin,AnthonyJ
烧伤创面和创面愈合引发了几个生物过程，这些过程可能会使光谱成像数据的解释复杂化。通过对使用分级猪烧伤创面愈合模型进行纵向调查获得的空间频域光谱数据(450到1000 nm)的分析，我们识别了吸收光谱中似乎表明存在血红蛋白分解产物的特征，例如高铁血红蛋白。我们的结果表明，高铁血红蛋白的计算浓度与烧伤后24小时的严重程度直接相关。此外，如果高铁血红蛋白不包括在光谱分析中，组织参数如氧合(StO2)和水分含量可能分别被低估20%和78%。
Burn wounds and wound healing invoke several biological processes that may complicate the interpretation of spectral imaging data. Through analysis of spatial frequency domain spectroscopy data (450 to 1000 nm) obtained from longitudinal investigations using a graded porcine burn wound healing model, we have identified features in the absorption spectrum that appear to suggest the presence of hemoglobin breakdown products, e.g., methemoglobin. Our results show that the calculated concentrations of methemoglobin directly correlate with burn severity, 24 h after the injury. In addition, tissue parameters such as oxygenation (StO2) and water fraction may be underestimated by 20% and 78%, respectively, if methemoglobin is not included in the spectral analysis.