Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus
Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus
复制标题
DOI：
10.1099/0022-1317-80-8-2031
复制
发表时间：
1999-08-01
影响因子：
3.8
通讯作者：
Barrett, T
中科院分区：
文献类型：
--
作者：
Baron, MD;Foster-Cuevas, M;Barrett, T
我们已经研究了牛的免疫反应，使用重组牛瘟病毒（RPV）表达的异源蛋白。在RPV疫苗株基因组的cDNA拷贝中创建了一个新的基因单位，并插入了一个开放阅读框，该阅读框编码聚合酶（3D（pol））和口蹄疫病毒（FMDV）衣壳蛋白VP 1的部分。感染性重组RPV被拯救，并显示在感染的细胞中以良好的水平表达FMDV衍生的蛋白。拯救的病毒在组织培养中仅比原始病毒稍微更减毒。感染该重组体的牛对RPV产生了正常的免疫应答，并保护其免受该病毒的致死性攻击。实验动物表现出特异性迟发型超敏反应FMDV 3D（波尔），类似于FMDV感染中所见;然而，没有检测到抗体识别FMDV衍生蛋白的任一组分，也没有任何增殖反应，这些表位发现在分离的外周血淋巴细胞从感染的动物。没有发现对FMDV感染的保护作用。
We have investigated the bovine immune response to heterologous proteins expressed using a recombinant rinderpest virus (RPV). A new gene unit was created in a cDNA copy of the genome of the vaccine strain of RPV, and an open reading frame inserted that encodes the polymerase (3D(pol)) and parts of the capsid protein VP1 from foot-and-mouth disease virus (FMDV). Infectious recombinant RPV was rescued and shown to express the FMDV-derived protein at good levels in infected cells. The rescued virus was only slightly more attenuated in tissue culture than the original virus. Cattle infected with this recombinant generated a normal immune response to RPV, and were protected from lethal challenge by that virus. Experimental animals showed a specific delayed-type hypersensitivity response to FMDV 3D(pol), similar to that seen in FMDV infection; however, no antibodies were detected recognizing either of the components of the FMDV-derived protein, nor was any proliferative response to these epitopes found in isolated peripheral blood lymphocytes from infected animals. No protection was seen against FMDV infection.