Association of cocaine- and amphetamine-regulated transcript (CART) messenger RNA level, food intake, and growth in channel catfish
Association of cocaine- and amphetamine-regulated transcript (CART) messenger RNA level, food intake, and growth in channel catfish
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DOI：
10.1016/j.cbpa.2008.06.029
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发表时间：
2008-10-01
影响因子：
2.3
通讯作者：
Waldbieser, Geoffrey C.
中科院分区：
文献类型：
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作者：
Kobayashi, Yasuhiro;Peterson, Brian C.;Waldbieser, Geoffrey C.
可卡因和苯丙胺调节转录物（CART）是哺乳动物和鱼类中一种有效的下丘脑厌食肽。我们假设，增加食物摄入量与CART mRNA在癍点叉尾鮰脑内表达的变化有关。目的是克隆CART基因，检测组织CART mRNA的分布，以及CART mRNA的量与癍点叉尾鮰摄食量变化的关系。我们的研究结果表明，海峡鲶鱼CART是高度相似的其他鱼类物种，特别是在生物活性部分的肽。CART mRNA在脑和睾丸中表达，但在其他躯体组织中未检测到。禁食30天后，
Cocaine-and Amphetamine-Regulated Transcript (CART) is a potent hypothalamic anorectic peptide in mammals and fish. We hypothesized that increased food intake is associated with changes in expression of CART mRNA within the brain of channel catfish. Objectives were to clone the CART gene, examine tissue CART mRNA distribution, and changes in the amount of CART mRNA in relation to changes in food intake in channel catfish. Our results showed that channel catfish CART was highly similar to those of other fish species, particularly in the biologically active portion of the peptide. Expression of CART mRNA was detected in the brain and testis but not in other somatic tissues. Thirty days of fasting decreased (P