The Long Non-Coding RNA RHPN1-AS1 Promotes Uveal Melanoma Progression.
The Long Non-Coding RNA RHPN1-AS1 Promotes Uveal Melanoma Progression.
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长非编码RNA RHPN1-AS1促进葡萄膜黑色素瘤进展
DOI：
10.3390/ijms18010226
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发表时间：
2017-01-23
影响因子：
5.6
通讯作者：
Fan X
中科院分区：
文献类型：
--
作者：
Lu L;Yu X;Zhang L;Ding X;Pan H;Wen X;Xu S;Xing Y;Fan J;Ge S;Zhang H;Jia R;Fan X
越来越多的证据表明，异常长链非编码RNA（lncRNA）与肿瘤的发生、发展和转移密切相关。RHPN 1反义RNA 1（RHPN 1-AS 1）是一个2030-bp的转录本，来源于人类染色体8 q24。然而，RHPN 1-AS 1在葡萄膜黑色素瘤（UM）中的作用仍有待澄清。本研究旨在阐明RHPN 1-AS 1在UM中的分子功能。采用实时荧光定量聚合酶链反应（qRT-PCR）检测UM细胞系中RHPN 1-AS 1的RNA水平。设计短干扰RNA（siRNA）抑制RHPN 1-AS 1的表达，建立稳定表达短发夹（sh）RHPN 1-AS 1的UM细胞。接着，使用集落形成测定和transwell迁移/侵袭测定来确定细胞增殖和迁移能力。建立了裸鼠移植瘤模型，以证实RHPN 1-AS 1在体内的功能。与正常人视网膜色素上皮（RPE）细胞系相比，RHPN 1-AS 1在许多UM细胞系中显著上调。RHPN 1-AS 1基因敲低可显著抑制UM细胞的增殖和迁移。我们的数据表明，RHPN 1-AS 1可能是UM中的一种癌RNA，其可能作为恶性UM的候选预后生物标志物和新治疗靶点。
Increasing evidence suggests that aberrant long non-coding RNAs (lncRNAs) are significantly correlated with the pathogenesis, development and metastasis of cancers. RHPN1 antisense RNA 1 (RHPN1-AS1) is a 2030-bp transcript originating from human chromosome 8q24. However, the role of RHPN1-AS1 in uveal melanoma (UM) remains to be clarified. In this study, we aimed to elucidate the molecular function of RHPN1-AS1 in UM. The RNA levels of RHPN1-AS1 in UM cell lines were examined using the quantitative real-time polymerase chain reaction (qRT-PCR). Short interfering RNAs (siRNAs) were designed to quench RHPN1-AS1 expression, and UM cells stably expressing short hairpin (sh) RHPN1-AS1 were established. Next, the cell proliferation and migration abilities were determined using a colony formation assay and a transwell migration/invasion assay. A tumor xenograft model in nude mice was established to confirm the function of RHPN1-AS1 in vivo. RHPN1-AS1 was significantly upregulated in a number of UM cell lines compared with the normal human retinal pigment epithelium (RPE) cell line. RHPN1-AS1 knockdown significantly inhibited UM cell proliferation and migration in vitro and in vivo. Our data suggest that RHPN1-AS1 could be an oncoRNA in UM, which may serve as a candidate prognostic biomarker and target for new therapies in malignant UM.