THE RAPID PREPARATION OF FROZEN TISSUE SECTIONS
THE RAPID PREPARATION OF FROZEN TISSUE SECTIONS
复制标题
DOI：
10.3109/10520294809106233
复制
发表时间：
1948-01-01
期刊：
影响因子：
--
通讯作者：
TAYLOR, AB
中科院分区：
文献类型：
--
作者：
ADAMSTONE, FB;TAYLOR, AB
该程序的基本特征包括切除后快速冷冻组织，并保持冷冻状态直至应用所需的化学物质或固定剂。对于冷冻，可以根据需要使用二氧化碳或液态空气。通过在其表面粘贴干冰块来彻底冷却切片机刀。切好的部分仍处于冷冻状态，用骆驼毛刷进行操作，使其平放在刀上。然后通过特殊的部分升降机将它们转移到滑梯上。它的形状是装满干冰的双底勺。因此，切片在转移到干净的显微镜载玻片时保持冷冻状态，该载玻片以一定角度固定在装有所需试剂的科普林罐上方。必须在熔化前将切片浸入水中。如果它们仍然僵硬地冷冻，它们会卷曲并且不会粘附在载玻片上，并且如果在融化后浸入，它们会变形。利用这种技术，可以获得显示最小细胞变形的切片。因此，它促进了许多细胞学技术、化学测试和酶学研究的使用，例如 Gomori 技术。组织。
The essential feature of the procedure involves the rapid freezing of the tissue following excision and keeping it frozen until the desired chemical or fixative has been applied. For freezing, either CO2 or liquid air is used, as desired. The microtome knife is thoroughly cooled by taping blocks of dry ice at its surface. The cut sections, still frozen, are manipulated by a camel''s hair brush so that they lie flat upon the knife. They are then transferred to a slide by a special section lifter. This has the form of a double-bottomed scoop packed with dry ice. Thus the section remains frozen while it is transferred to a clean microscope slide held at an angle above a Coplin jar of the desired reagent. The sections must be immersed just prior to melting. They curl and do not adhere to the slide if still rigidly frozen, and are distorted if immersed after melting. With this technic sections showing a minimum of cellular distortion may be obtained. Consequently, it facilitates the use of many cytological technics, chemical tests, and enzymatic studies, such as the Gomori technics, on a var. of tissues.