Exploring genetic variation for salinity tolerance in chickpea using image-based phenotyping.
Exploring genetic variation for salinity tolerance in chickpea using image-based phenotyping.
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DOI：
10.1038/s41598-017-01211-7
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发表时间：
2017-05-02
影响因子：
4.6
通讯作者：
Sutton T
中科院分区：
文献类型：
--
作者：
Atieno J;Li Y;Langridge P;Dowling K;Brien C;Berger B;Varshney RK;Sutton T
土壤盐碱化导致鹰嘴豆生产力降低。然而，耐盐育种是具有挑战性的，因为有限的知识的关键性状影响高盐下的性能和高通量表型的困难，大，多样的种质资源收集。本研究利用基于图像的表型研究鹰嘴豆耐盐性的遗传变异在245个不同的加入。平均盐度降低植物生长速率（通过跟踪叶片扩展时间）的20%，植物高度的15%和拍摄生物量的28%。另外，盐胁迫导致角果败育，抑制角果充实，使种子数和种子产量分别减少16%和32%。重要的是，观察到中度至强相关性的不同性状之间测量的温室和两个领域的网站，表明温室测定相关的领域的表现。使用基于图像的表型分析，我们测量了植物的生长速度在盐度和随后阐明了拍摄离子独立的压力（导致液压阻力和渗透胁迫）在鹰嘴豆的作用。在多样性小组中观察到耐盐性的广泛遗传变异，种子数量是耐盐性的主要决定因素。本研究提出种子数可作为筛选耐盐鹰嘴豆品种的一个选择性状。
Soil salinity results in reduced productivity in chickpea. However, breeding for salinity tolerance is challenging because of limited knowledge of the key traits affecting performance under elevated salt and the difficulty of high-throughput phenotyping for large, diverse germplasm collections. This study utilised image-based phenotyping to study genetic variation in chickpea for salinity tolerance in 245 diverse accessions. On average salinity reduced plant growth rate (obtained from tracking leaf expansion through time) by 20%, plant height by 15% and shoot biomass by 28%. Additionally, salinity induced pod abortion and inhibited pod filling, which consequently reduced seed number and seed yield by 16% and 32%, respectively. Importantly, moderate to strong correlation was observed for different traits measured between glasshouse and two field sites indicating that the glasshouse assays are relevant to field performance. Using image-based phenotyping, we measured plant growth rate under salinity and subsequently elucidated the role of shoot ion independent stress (resulting from hydraulic resistance and osmotic stress) in chickpea. Broad genetic variation for salinity tolerance was observed in the diversity panel with seed number being the major determinant for salinity tolerance measured as yield. This study proposes seed number as a selection trait in breeding salt tolerant chickpea cultivars.