PROMOTERS LARGELY DETERMINE THE EFFICIENCY OF REPRESSOR ACTION
PROMOTERS LARGELY DETERMINE THE EFFICIENCY OF REPRESSOR ACTION
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DOI：
10.1073/pnas.85.23.8973
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
BUJARD, H
中科院分区：
文献类型：
--
作者：
LANZER, M;BUJARD, H
操作序列和抑制蛋白对lac启动子活性的调控范围超过100 ~ 1000倍。然而，lac操作符与其他启动子序列的组合在抑制水平上差别很大。所提供的数据表明，抑制的程度在很大程度上取决于相互竞争的系统——操作子-抑制子和启动子-RNA聚合酶的复杂形成速率，以及RNA聚合酶清除启动子的速率。此外，当操作员被放置在启动子序列的不同位置时，发现抑制水平的差异高达70倍。提出了一个动力学模型来解释观察到的效应，并允许对由负作用元素控制的启动子进行预测。
Operator sequence and repressor protein regulate the activity of the lac promoter over a > 1000-fold range. Combinations of the lac operator with other promoter sequences, however, differ vastly in the level of repression. The data presented show that the extent of repression is determined largely by the rates of complex formation of the competing systems operator-repressor and promoter-RNA polymerase and by the rate at which RNA polymerase clears the promoter. Moreover, up to 70-fold differences in the level of repression were found when the operator was placed in different positions within the promoter sequence. A kinetic model is proposed that explains the observed effects and that allows predictions on promoters controlled by negatively acting elements.