Surface grafting of zwitterionic polymers onto dye doped AIE-active luminescent silica nanoparticles through surface-initiated ATRP for biological imaging applications
Surface grafting of zwitterionic polymers onto dye doped AIE-active luminescent silica nanoparticles through surface-initiated ATRP for biological imaging applications
复制标题
通过表面引发的 ATRP 将两性离子聚合物表面接枝到染料掺杂的 AIE 活性发光二氧化硅纳米颗粒上，用于生物成像应用
DOI：
10.1016/j.apsusc.2017.05.041
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发表时间：
2017-10-15
影响因子：
6.7
通讯作者：
Wei, Yen
中科院分区：
文献类型：
--
作者：
Mao, Liucheng;Liu, Xinhua;Wei, Yen
与传统有机染料相比，聚集诱导发射(ME)染料具有优异的光学特性，近年来在生物成像领域得到了广泛的应用。AIE活性发光二氧化硅纳米颗粒(LSNPs)有望结合二氧化硅纳米颗粒和AIE活性染料的优点。虽然以前已经制备了具有AIE活性的LSNPs，但到目前为止还没有用功能聚合物对这些AIE活性的LSNPs进行表面修饰的报道。在这项工作中，我们报告了一种相当简单和通用的策略，通过表面引发的原子转移自由基聚合(ATRP)来制备具有AIE活性的聚合物LSNP5。通过非共价修饰的Stober方法将AIE活性染料直接包裹到二氧化硅纳米颗粒中，制备了AIE活性的LSNPs。通过3-氨丙基三乙氧基硅烷和2-溴异丁基溴的酰胺化反应，将ATRP引发剂固定在这些具有AIE活性的LSNPs上。最后，选择两性离子的2-(甲基丙烯酰氧基)乙基磷酰胆碱(MPC)作为模型单体，通过ATRP将其接枝到MSN上。表征结果表明，通过表面引发ATRP，聚(MPC)可以成功地对LSNP5进行改性。生物学评价结果表明，最终得到的SNPs-AIE-pMPC复合材料具有较低的细胞毒性、良好的光学性能和很大的生物成像潜力。综上所述，我们证明了AIE活性的LSNP5可以被制备并用功能聚合物进行表面修饰，从而赋予生物医学应用新的功能和更好的性能。更重要的是，由于ATRP具有良好的单体适应性，这一策略也可以推广到许多其他LSNPs聚合物复合材料的制备。(C)2017爱思唯尔B.V.保留所有权利。
Aggregation-induced emission (ME) dyes have recently been intensively explored for biological imaging applications owing to their outstanding optical feature as compared with conventional organic dyes. The AIE-active luminescent silica nanoparticles (LSNPs) are expected to combine the advantages both of silica nanoparticles and AIE-active dyes. Although the AIE-active LSNPs have been prepared previously, surface modification of these AIE-active LSNPs with functional polymers has not been reported thus far. In this work, we reported a rather facile and general strategy for preparation of polymers functionalized AIE-active LSNP5 through the surface-initiated atom transfer radical polymerization (ATRP). The AIE-active LSNPs were fabricated via direct encapsulation of AIE-active dye into silica nanoparticles through a non covalent modified Stober method. The ATRP initiator was subsequently immobilized onto these AIE-active LSNPs through amidation reaction between 3-aminopropyl-triethoxy-silane and 2-bromoisobutyryl bromide. Finally, the zwitterionic 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) was selected as model monomer and grafted onto MSNs through ATRP. The characterization results suggested that LSNP5 can be successfully modified with poly(MPC) through surface-initiated ATRP. The biological evaluation results demonstrated that the final SNPs-AIE-pMPC composites possess low cytotoxicity, desirable optical properties and great potential for biological imaging. Taken together, we demonstrated that AIE-active LSNP5 can be fabricated and surface modified with functional polymers to endow novel functions and better performance for biomedical applications. More importantly, this strategy developed in this work could also be extended for fabrication of many other LSNPs polymer composites owing to the good monomer adoptability of ATRP. (C) 2017 Elsevier B.V. All rights reserved.