Pathological Impact on the Phyllosphere Microbiota of Artemisia argyi by Haze.
Pathological Impact on the Phyllosphere Microbiota of Artemisia argyi by Haze.
复制标题
雾霾对艾叶叶际微生物群的病理影响
DOI：
10.4014/jmb.2009.09024
复制
发表时间：
2021-04-28
影响因子：
2.8
通讯作者：
Wei-Jun, Ding
中科院分区：
文献类型：
--
作者：
Yu-Zhu, Zhang;De-Yu, Jiang;Chi, Zhang;Kun, Yang;Huai-Fu, Wang;Xiu-Wen, Xia;Wei-Jun, Ding
霾对叶际微生物群的病理影响有待研究。在中国成都选择中度霾环境和清洁对照。同时选择分布广泛、应用广泛的中草药艾蒿作为实验材料。从叶样品中提取总基因组DNA，并且对于宏基因组测序，应用Illumina HiSeq 2500平台。结果表明，灰霾叶片叶际微生物区系基因数低于清洁对照。雾霾组和清洁组叶际微生物区系的前10门相同，雾霾组变形菌、放线菌和异球藻的丰度显著增加，而子囊菌和担子菌的丰度减少。在属的水平上，烟霾叶片中诺卡氏菌、副球菌、大理石生菌和克氏菌的丰度显著增加，而酵母菌的丰度则显著减少。KEGG检索结果显示，功能基因大多与代谢相关。这项工作的一个有趣的发现是，叶际微生物群负责合成初级和次级代谢产物在A。在灰霾环境下，土壤中的砷含量显著增加。eggNOG注释的基因相对富集，属于复制、重组和修复，属于糖苷水解酶和糖基转移酶的基因显著增加。总之，我们发现叶际微生物群的结构和功能都受到雾霾的全面影响，而负责雾霾耐受性的初级和次级代谢产物显着增加。这些结果表明，植物的适应性策略，以容忍和面对烟雾损害。
The pathological impact of haze upon the phyllosphere microbiota awaits investigation. A moderate degree of haze environment and a clean control were selected in Chengdu, China. Artemisia argyi, a ubiquitously distributed and extensively applied Chinese herb, was also chosen for experiment. Total genome DNA was extracted from leaf samples, and for metagenome sequencing, an Illumina HiSeq 2500 platform was applied. The results showed that the gene numbers of phyllosphere microbiota derived from haze leaves were lower than those of the clean control. The phyllosphere microbiota derived from both haze and clean groups shared the same top ten phyla; the abundances of Proteobacteria, Actinomycetes and Anorthococcuso of the haze group were substantially increased, while Ascomycetes and Basidiomycetes decreased. At the genus level, the abundances of Nocardia, Paracoccus, Marmoricola and Knoelia from haze leaves were markedly increased, while the yeasts were statistically decreased. KEGG retrieval demonstrated that the functional genes were most annotated to metabolism. An interesting find of this work is that the phyllosphere microbiota responsible for the synthesis of primary and secondary metabolites in A. argyi were significantly increased under a haze environment. Relatively enriched genes annotated by eggNOG belong to replication, recombination and repair, and genes classified into the glycoside hydrolase and glycosyltransferase enzymes were significantly increased. In summary, we found that both structure and function of phyllosphere microbiota are globally impacted by haze, while primary and secondary metabolites responsible for haze tolerance were considerably increased. These results suggest an adaptive strategy of plants for tolerating and confronting haze damage.