Vitreous levels of vascular endothelial growth factor are not influenced by its serum concentrations in diabetic retinopathy
Vitreous levels of vascular endothelial growth factor are not influenced by its serum concentrations in diabetic retinopathy
复制标题
DOI：
10.1007/s001250050794
复制
发表时间：
1997-09-01
期刊：
影响因子：
8.2
通讯作者：
Carrascosa, A
中科院分区：
文献类型：
--
作者：
Burgos, R;Simo, R;Carrascosa, A
血管内皮生长因子（VEGF）在增殖性糖尿病视网膜病变（PDR）的新生血管形成中起主要作用。玻璃体内VEGF的来源可能是缺血性视网膜，但不能排除来自血清的水平升高。本研究的目的是测定糖尿病合并PDR患者玻璃体内VEGF的浓度，并探讨血清VEGF是否与玻璃体内VEGF浓度有关。为此目的，我们研究了20例糖尿病患者（5例IDDM和15例NIDDM）伴PDR，其中进行了玻璃体切除术（A组）。非糖尿病患者（n = 13）与其他条件需要玻璃体切除术作为对照组（组B）。在这两组中，VEGF测定血清和未稀释的玻璃体样本同时获得。同时检测69例健康对照者（C组）和39例无微血管并发症的糖尿病患者（D组）血清VEGF水平。通过ELISA（R & D Systems，阿宾登，英国）测定维生素D和血清VEGF;测定内CV 3.8%，测定间CV 5.1%。与B组（中位数0.009 ng/ml，范围0.009-0.038）相比，A组玻璃体内VEGF浓度（中位数1.75 ng/ml，范围0.33-6.66）显著更高; p < 0.0001。在调整玻璃体内蛋白浓度后，该差异仍然显著（p < 0.05）。血清VEGF的差异包括在研究中的各组之间没有founded. We的结论是，高玻璃体水平的血管内皮生长因子在糖尿病患者与PDR不能归因于血清扩散穿过血视网膜屏障。因此，眼内合成是PDR中观察到的高玻璃体VEGF浓度的主要促成因素。
Vascular endothelial growth factor (VEGF) plays a major role in the development of neovascularization in proliferative diabetic retinopathy (PDR). The source of intravitreous VEGF is presumably ischaemic retina, but increased levels derived from serum cannot be excluded. The aim of the study is to determine the intravitreous concentrations of VEGF in diabetic patients with PDR and to investigate whether serum VEGF could contribute to the intravitreous concentration. For this purpose, we studied 20 diabetic patients (5 IDDM and 15 NIDDM) with PDR in whom a vitrectomy was performed (group A). Non-diabetic patients (n = 13) with other conditions requiring vitrectomy served as a control group (group B). In both groups, VEGF was determined in serum and undiluted vitreous samples obtained simultaneously. Furthermore, serum VEGF was determined in 69 healthy control subjects (group C) and 39 diabetic patients without microvascular complications (group D). Vitreous and serum VEGF was determined by ELISA (R & D Systems, Abingdon, UK); intra-assay CV 3.8%, interassay CV 5.1%. Intravitreous concentrations of VEGF were strikingly higher in group A (median 1.75 ng/ ml, range 0.33-6.66) in comparison with group B (median 0.009 ng/ml, range 0.009-0.038); p < 0.0001. This difference remained significant after adjusting for intravitreous protein concentration (p < 0.05). Differences in serum VEGF among the groups included in the study were not found. We conclude that the high vitreous levels of VEGF observed in diabetic patients with PDR cannot be attributed to serum diffusion across the blood-retinal barrier. Therefore, intraocular synthesis is the main contributing factor for the high vitreous VEGF concentrations observed in PDR.