HDL cholesterol quantitation by phosphotungstate-Mg2+ and by dextran sulfate-Mn2+-polyethylene glycol precipitation, both with enzymic cholesterol assay compared with the lipid research method.
HDL cholesterol quantitation by phosphotungstate-Mg2+ and by dextran sulfate-Mn2+-polyethylene glycol precipitation, both with enzymic cholesterol assay compared with the lipid research method.
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采用磷钨酸盐-Mg2+和硫酸葡聚糖-Mn2+-聚乙二醇沉淀法定量HDL胆固醇，均与酶法胆固醇测定法与脂质研究方法进行比较。
DOI：
10.1093/ajcp/78.5.718
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发表时间：
1982
影响因子：
3.5
通讯作者：
Albers,JJ
中科院分区：
文献类型：
--
作者：
Warnick,GR;Mayfield,C;Benderson,J;Chen,JS;Albers,JJ
将两种使用商业试剂盒进行高密度脂蛋白（HDL）胆固醇定量的方法与脂质研究诊所（LRC）程序进行比较。采用Lancer HDL胆固醇快速统计试剂盒（Lancer）磷钨酸盐-Mg 2+沉淀和酶法胆固醇测定法对50例患者标本进行HDL胆固醇定量，平均值为424 mg/L，采用硫酸葡聚糖-Mn 2 +-聚乙二醇（硫酸葡聚糖）沉淀和酶法胆固醇测定法，平均值为474 mg/L。相比之下，LRC方法（肝素-Mn 2+沉淀结合Liebermann-Burchard试剂胆固醇测定）的平均值为478 mg/L。当通过LRC试验分析时，通过三种沉淀方法获得的上清液具有相似的胆固醇值，表明观察到的差异主要是由于胆固醇试验之间的差异。结果与上清液中胆固醇的酶法测定低估一致，由硫酸葡聚糖产生的上清液中Mn 2+的正干扰抵消。观察到Lancer方法的日间CV为4-5%，硫酸葡聚糖方法的日间CV为6-7%。这两种方法都能很好地沉积hyperoxidemic标本中的沉淀物。
Two methods using commercial kits for high density lipoprotein (HDL) cholesterol quantitation were compared with the Lipid Research Clinics (LRC) procedures. HDL cholesterol quantitations on 50 patient specimens by the Lancer HDL cholesterol Rapid Stat Kit (Lancer) with phosphotungstate-Mg2+precipitation and enzymic cholesterol assay averaged 424 mg/L, and by a method with dextran sulfate-Mn2+-polyethylene glycol (dextran sulfate) precipitation and enzymic cholesterol assay averaged 474 mg/L. By comparison, the LRC method (heparin-Mn2+precipitation combined with a Liebermann-Burchard reagent cholesterol assay) averaged 478 mg/L. Supernates obtained by the three precipitation methods had similar cholesterol values when analyzed by the LRC assay, suggesting that the observed differences were primarily due to differences between the cholesterol assays. Results were consistent with underestimation by the enzymic assay of cholesterol in the supernates, offset by a positive interference of Mn2+in the dextran sulfate-produced supernates. Among-day CVs of 4–5% were observed for the Lancer method, and 6–7% for the dextran sulfate method. Sedimentation of precipitates in hypertriglyceridemic specimens was excellent by both methods.