Crossover interference is abolished in the absence of a synaptonemal complex protein
Crossover interference is abolished in the absence of a synaptonemal complex protein
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DOI：
10.1016/0092-8674(94)90197-x
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发表时间：
1994-10
期刊：
影响因子：
64.5
通讯作者：
M. Sym;G. Roeder
中科院分区：
文献类型：
--
作者：
M. Sym;G. Roeder
在zip 7突变体中，由于联会复合体（SC）的结构成分的缺乏，减数分裂染色体不能联会。该突变体已被分析的能力进行了几个功能，已提出的SC。所提供的数据表明，zip 1突变不影响交叉功能，并赋予只有适度的缺陷，在减数分裂重组和姐妹染色单体凝聚力。相比之下，交叉干扰在Zipl不存在的情况下被完全消除。这些数据是第一个建立SC的细胞学观察和遗传干扰现象之间的分子联系。联会复合体（SC）是在减数分裂期间沿同源染色体长度沿着发现的蛋白质的精细大分子组装体（vonWettstein等，1984年）。减数分裂前DNA复制之后是前期I期间的一系列事件，将同源染色体聚集在一起并促进遗传重组。在酵母中，SC形态发生中最早可检测的中间体可以被视为称为轴向元件的蛋白质核心的短延伸，其沿着每对姐妹染色单体形成沿着（梳妆台和Giroux，1966; Alani等人，1990年）。随着这些芯的伸长，在SC的上下文中，同源轴向元件之间的紧密关联开始形成。一旦轴向元件组装到SC中，它们就被称为侧向元件。两个横向元件之间的区域被称为中心区域。在前期I的粗线期，每对同源物完全突触，SC呈现为沿着每对染色体全长的带状结构。
In the zip7 mutant, meiotic chromosomes fail to synapse, owing to the absence of a structural component of the synaptonemal complex (SC). This mutant has been analyzed for the ability to carry out several functions that have been proposed for the SC. The data presented show that the zip1 mutation does not affect chiasma function and confers only modest defects in meiottc recombination and sister chromatid cohesion. In contrast, crossover interference is completely abolished in the absence of Zipl. These data are the first to establish a molecular link between cytological observations of the SC and the genetic phenomenon of interference. introductionThe synaptonemal complex (SC) is an elaborate macromolecular assembly of proteins found along the lengths of homologous chromosomes during meiosis (von Wettstein et al., 1984). Premeiotic DNA replication is followed by a series of events during prophase I that brings homologous chromosomes together and promotes genetic recombination. In yeast, the earliest detectable intermediates in SC morphogenesis can be seen as short stretches of protein cores called axial elements that form along each pair of sister chromatids (Dresser and Giroux, 1966; Alani et al., 1990). As these cores elongate, intimate associations between homologous axial elements begin to form in the context of SC. Once axial elements are assembled into the SC, they are referred to as lateral elements. The area between two lateral elements is referred to as the central region. During the pachytene stage of prophase I, each pair of homologs is fully synapsed, and the SC appears as a ribbon-like structure along the entire length of each chromosome pair.