Prenatal exposure to zinc oxide particles alters monoaminergic neurotransmitter levels in the brain of mouse offspring
Prenatal exposure to zinc oxide particles alters monoaminergic neurotransmitter levels in the brain of mouse offspring
复制标题
DOI：
10.2131/jts.38.363
复制
发表时间：
2013-06-01
影响因子：
2
通讯作者：
Takeda, Ken
中科院分区：
文献类型：
--
作者：
Okada, Yuka;Tachibana, Ken;Takeda, Ken
氧化锌（ZnO）纳米颗粒（NPs）是用于防晒霜和化妆品的有益材料。虽然氧化锌纳米粒子广泛用于化妆品，但在怀孕期间接触氧化锌对后代的健康影响在很大程度上是未知的。本文研究了产前暴露于氧化锌NPs对小鼠大脑单胺能系统的影响。采用高效液相色谱法（HPLC）对妊娠期ICR小鼠皮下注射氧化锌NPs（总剂量为500 μ g/只），测定6周龄后代大脑9个区域多巴胺（DA）、血清素（5-HT）、去甲肾上腺素及其代谢物的含量。HPLC分析显示，ZnO NP暴露组海马DA水平升高。在DA代谢物水平上，产前ZnO NP暴露使前额皮质和海马中的高香草酸增加，前额皮质中的3,4 -二羟基苯乙酸增加。此外，氧化锌NP暴露组前额皮质、新纹状体、伏隔核和杏仁核的DA转换水平升高。我们还发现ZnO np暴露组下丘脑血清素水平以及前额皮质和海马中5-HIAA （5-HT代谢物）水平的变化。产前氧化锌NP暴露使5-羟色胺在除小脑外的所有区域均升高。本研究表明，产前暴露于氧化锌NPs可能会破坏单胺能系统，并可能对后代的心理健康产生不利影响。
Zinc oxide (ZnO) nano-sized particles (NPs) are beneficial materials used for sunscreens and cosmetics. Although ZnO NPs are widely used for cosmetics, the health effects of exposure during pregnancy on offspring are largely unknown. Here we investigated the effects of prenatal exposure to ZnO NPs on the monoaminergic system of the mouse brain. Subcutaneous administration of ZnO NPs to the pregnant ICR mice (total 500 mu g/mouse) were carried out and then measured the levels of dopamine (DA), serotonin (5-HT), and noradrenalin, and their metabolites in 9 regions of the brain of offspring (6-week-old) using high performance liquid chromatography (HPLC). HPLC analysis demonstrated that DA levels were increased in hippocampus in the ZnO NP exposure group. In the levels of DA metabolites, homovanillic acid was increased in the prefrontal cortex and hippocampus, and 3, 4-dihydroxy-phenylacetic acid was increased in the prefrontal cortex by prenatal ZnO NP exposure. Furthermore, DA turnover levels were increased in the prefrontal cortex, neostriatum, nucleus accumbens, and amygdala in the ZnO NP exposure group. We also found changes of the levels of serotonin in the hypothalamus, and of the levels of 5-HIAA (5-HT metabolite) in the prefrontal cortex and hippocampus in the ZnO NP-exposed group. The levels of 5-HT turnover were increased in each of the regions except for the cerebellum by prenatal ZnO NP exposure. The present study indicated that prenatal exposure to ZnO NPs might disrupt the monoaminergic system, and suggested the possibility of detrimental effects on the mental health of offspring.