PtDRG1, a Desiccation Response Gene from Pyropia tenera (Rhodophyta), Exhibits Chaperone Function and Enhances Abiotic Stress Tolerance
PtDRG1, a Desiccation Response Gene from Pyropia tenera (Rhodophyta), Exhibits Chaperone Function and Enhances Abiotic Stress Tolerance
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DOI：
10.1007/s10126-018-9828-2
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发表时间：
2018-05
影响因子：
3
通讯作者：
Yeonju Na;Ha-Nul Lee;Jiwoong Wi;W. Jeong;D. Choi
中科院分区：
文献类型：
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作者：
Yeonju Na;Ha-Nul Lee;Jiwoong Wi;W. Jeong;D. Choi
鹿蹄草是有商业价值的海洋红藻，生长在潮间带。它们对干燥胁迫具有极强的耐受性。我们以前已经确定和报告的脱水反应基因（DRG）的基础上转录组分析的P。其中PtDRG 1编码一个分子量为22.6kDa的多肽，定位于叶绿体中。PtDRG1与公共数据库中保存的任何已知基因都不具有序列同源性。PtDRG 1基因的转录水平在甘露醇和H2O2诱导的渗透胁迫以及干燥胁迫下均被上调，而在高温胁迫下则没有上调。当PtDRG 1在大肠杆菌或衣原体中过量表达时，转化细胞在高温以及甘露醇和NaCl诱导的渗透胁迫下的生长明显优于对照细胞。此外，PtDRG 1显着减少热应激条件下底物蛋白的热聚集。这些结果表明PtDRG 1具有分子伴侣功能，在植物抗非生物胁迫机制中发挥重要作用。这项研究表明，红藻有未知的应激蛋白，如PtDRG 1，有助于胁迫耐受性。
Pyropiaare commercially valuable marine red algae that grow in the intertidal zone. They are extremely tolerant to desiccation stress. We have previously identified and reported desiccation response genes (DRGs) based on transcriptome analysis ofP. tenera.Among them,PtDRG1encodes a polypeptide of 22.6 kDa that is located in the chloroplast. PtDRG1 does not share sequence homology with any known gene deposited in public database. Transcription ofPtDRG1gene was upregulated by osmotic stress induced by mannitol or H2O2as well as desiccation stress, but not by heat. WhenPtDRG1was overexpressed inEscherichia coliorChlamydomonas, transformed cells grew much better than control cells under high temperature as well as osmotic stress induced by mannitol and NaCl. In addition, PtDRG1 significantly reduced thermal aggregation of substrate protein under heat stress condition. These results demonstrate that PtDRG1 has a chaperone function and plays a role in tolerance mechanism for abiotic stress. This study shows that red algae have unknown stress proteins such as PtDRG1 that contributes to stress tolerance.