Phylogenetic implications of nuclear rRNA IGS variation in Stipa L. (Poaceae)
Phylogenetic implications of nuclear rRNA IGS variation in Stipa L. (Poaceae)
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DOI：
10.1038/s41598-017-11804-x
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发表时间：
2017-09-14
影响因子：
4.6
通讯作者：
Sawicki, Jakub
中科院分区：
文献类型：
--
作者：
Krawczyk, Katarzyna;Nobis, Marcin;Sawicki, Jakub
本文讨论了分子标记缺乏的问题，该标记可以说明针茅属（禾本科）内的分子变异性以及系统发育关系。迄今为止所做的研究尚未提供有关针茅属特定分类群之间关系的足够信息。在本研究中，我们分析了该属的六个物种与禾本科其他五个物种的核核糖体 DNA 的变异性和系统发育信息，包括将该区域划分为功能元件和域。我们的结果表明，基因间间隔区，特别是其与 26 S nrDNA 相邻的部分，是一个分子标记，为针茅的系统发育重建提供了真正的机会。从迄今为止测试的所有叶绿体和核标记来看，该区域似乎是针茅的系统发育信息最丰富的区域。对针茅和禾本科其他代表的 nrDNA 重复单元的比较分析表明，它们的结构没有偏离一般方案。然而，IGS 区域重复序列内的进化速率非常高，因此它注定了该区域可以在属水平或更浅的分类尺度上进行针茅的系统发育分析。
The article takes up the problem of deficiency of molecular marker, which could illustrate molecular variability as well as phylogenetic relation within the genus of Stipa L. (Poaceae). Researches made so far hadn't delivered sufficient information about relationships between particular taxa from the genus of Stipa. In the present study, we analyzed variability and phylogenetic informativeness of nuclear ribosomal DNA in six species from the genus against five other species from Poaceae including a division of this region into functional elements and domains. Our results showed that the intergenic spacer region, and especially its part adjacent to 26 S nrDNA, is a molecular marker giving a real chance for a phylogeny reconstruction of Stipa. The region seems to be the most phylogenetically informative for Stipa from all the chloroplast and nuclear markers tested so far. Comparative analysis of nrDNA repeat units from Stipa to other representatives of Poaceae showed that their structure does not deviate from the general scheme. However, the rate of evolution within the inter-repeats in the IGS region is extremely high and therefore it predestines the region for phylogenetic analyses of Stipa at genus level or in shallower taxonomic scale.