Direct Conversion of Human Pluripotent Stem Cells to Osteoblasts With a Small Molecule.
Direct Conversion of Human Pluripotent Stem Cells to Osteoblasts With a Small Molecule.
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DOI：
10.1002/cpsc.44
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发表时间：
2018-02-28
影响因子：
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通讯作者：
Varghese S
中科院分区：
文献类型：
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作者：
Kang H;Shih YV;Varghese S
人类多能干细胞(HPSCs)具有无限的自我更新能力，可分化为人体内所有类型的细胞，是一种很有前途的细胞治疗和再生医学来源。由于其成本效益和可扩展性，小分子在从hPSCs中获得组织特异性细胞方面具有巨大的潜力。在这里，我们描述了一种通过使用单一的、自然的小分子腺苷从hPSCs获得成骨细胞的方案。该方案提供了一种简单的实验方案，可以从hPSCs中获得大量具有形成功能骨组织能力的成骨细胞或骨祖细胞。
Human pluripotent stem cells (hPSCs) that exhibit unlimited self-renewal ability and differentiate into all cell types in the human body are a promising cell source for cell based therapies and regenerative medicine. Small molecules hold great potential in deriving tissue-specific cells from hPSCs owing to their cost-effectiveness and scalability. Here, we describe a protocol of deriving osteoblasts from hPSCs by using a single, natural small molecule, adenosine. This protocol offers a simple experimental protocol to obtain large numbers of osteoblasts or osteoprogenitor cells from hPSCs with the ability to form functional bone tissues.