Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila
Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila
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DOI：
10.1016/j.cell.2005.12.017
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发表时间：
2006-01-13
期刊：
影响因子：
64.5
通讯作者：
Kunes, S
中科院分区：
文献类型：
--
作者：
Ashraf, SI;McLoon, AL;Kunes, S
持久的记忆形式需要蛋白质合成，但合成模式与记忆存储的关系尚未确定。在这里，我们表明，神经活动指导果蝇钙/钙调蛋白依赖性激酶II（CaMKII）的mRNA，突触后位点，在那里它被迅速翻译。CaMKII合成的这些特征在长期记忆的诱导过程中被重现，并产生特定于记忆的局部蛋白质合成模式。我们表明，mRNA的运输和突触蛋白的合成是由RISC通路的组成部分，包括SDE 3解旋酶阿米蒂奇，这是特别需要的持久的记忆。阿米蒂奇定位于突触，并以与突触蛋白质合成模式呈负相关的记忆特异性模式丢失。因此，我们提出RISC通路的降解控制是与稳定记忆相关的突触蛋白合成模式的基础。
Long-lasting forms of memory require protein synthesis, but how the pattern of synthesis is related to the storage of a memory has not been determined. Here we show that neural activity directs the mRNA of the Drosophila Ca2+ Calcium/Calmodulin-dependent Kinase II (CaMKII), to postsynaptic sites, where it is rapidly translated. These features of CaMKII synthesis are recapitulated during the induction of a long-term memory and produce patterns of local protein synthesis specific to the memory. We show that mRNA transport and synaptic protein synthesis are regulated by components of the RISC pathway, including the SDE3 helicase Armitage, which is specifically required for long-lasting memory. Armitage is localized to synapses and lost in a memory-specific pattern that is inversely related to the pattern of synaptic protein synthesis. Therefore, we propose that degradative control of the RISC pathway underlies the pattern of synaptic protein synthesis associated with a stable memory.