Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs.
Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs.
复制标题
雄性小鼠生殖器官中微粒体 PGE 合酶 1 和环氧合酶的免疫组织化学定位。
作者：
M. Lazarus;C. J. Munday;N. Eguchi;S. Matsumoto;G. Killian;B. Kubata;Y. Urade
本文研究了微粒体前列腺素E合成酶-1(mPGES-1)和环氧合酶(COX)-1和-2在雄性小鼠生殖器官中的组织分布和细胞定位。Northern印迹显示，mPGES-1mRNA在附睾处表达较强，在肺、脾、皮肤、肾、结肠和脑中表达较弱。Northern印迹、RT-PCR和Western印迹结果显示，在雄性生殖道中，mPGES-1的表达从睾丸到附睾尾逐渐增加，在输精管中的表达最高。免疫组织化学染色显示，mPGES-1在睾丸间质细胞、附睾上皮细胞、输精管上皮细胞和精囊中均有表达。在附睾头、尾部和输精管中，COX-1的表达和免疫反应呈进行性增加，而COX-2主要在输精管表达。COX-1定位于附睾头、体部、尾部及输精管上皮细胞，COX-2定位于附睾尾及输精管远端上皮细胞。这些结果表明，mPGES-1与COX-1和COX-2协同表达，并参与男性生殖器官中PGE(2)的产生。
We investigated the tissue distribution and cellular localization of microsomal PGE synthase-1 (mPGES-1) and cyclooxygenase (COX)-1 and -2 in the male mouse reproductive organs. Northern blotting revealed that the mPGES-1 mRNA was expressed intensely in the epididymis and weakly in the lung, spleen, skin, kidney, colon, and brain. In the male reproductive tract, the expression of mPGES-1 increased from the testis to the cauda epididymis and was highest in the vas deferens when examined by Northern blotting, RT-PCR, and Western blotting. By immunohistochemistry, mPGES-1 was detected in Leydig cells of the testis and in epithelial cells of the epididymis, vas deferens, and seminal vesicles. In addition, the caput and cauda regions of the epididymis and the vas deferens in this order showed a progressive increase in the expression of COX-1 mRNA and immunoreactivity, whereas COX-2 was dominantly expressed in the vas deferens. COX-1 was localized in epithelial cells of the caput, corpus and cauda epididymis and of the vas deferens, and COX-2 was evident in epithelial cells of the distal cauda epididymis and vas deferens. These results show that mPGES-1 is expressed coordinately with COX-1 and COX-2 and is involved in PGE(2) production in male genital organs.
影响因子：
15.9
作者：
HARRIS, RC;MCKANNA, JA;BREYER, MD
通讯作者：
BREYER, MD
支持细胞分泌的蛋白质在体外刺激纯化的大鼠 Leydig 细胞中的 DNA 合成。
DOI：
10.1530/jrf.0.0900093
发表时间：
1990
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Ojeifo,JO;Byers,SW;Papadopoulos,V;Dym,M
通讯作者：
Dym,M