Adenovirus-mediated PDCD5 gene transfer sensitizes K562 cells to apoptosis induced by idarubicin in vitro and in vivo
Adenovirus-mediated PDCD5 gene transfer sensitizes K562 cells to apoptosis induced by idarubicin in vitro and in vivo
复制标题
腺病毒介导的 PDCD5 基因转移使 K562 细胞对伊达比星体外和体内诱导的细胞凋亡敏感
DOI：
10.1007/s10495-008-0206-9
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发表时间：
2008-05-01
期刊：
影响因子：
7.2
通讯作者：
Huang, Xiao-Jun
中科院分区：
文献类型：
--
作者：
Ruan, Guo-Rui;Zhao, Hong-Shan;Huang, Xiao-Jun
PDCD5（程序性细胞死亡5）加速某些肿瘤细胞的凋亡，在AML和CML患者的骨髓有核细胞中低水平表达。在本研究中，我们评估了PDCD5过表达对白血病细胞药物敏感性的影响。用idarubicin （IDR）单独或与表达PDCD5的腺病毒载体（Ad-PDCD5）联合治疗K562细胞。annexin-V-FITC/PI双标记显示，与IDR单独治疗相比，Ad-PDCD5联合治疗后细胞凋亡率明显增加。我们观察到PDCD5过表达可显著提高体内低剂量IDR治疗的抗肿瘤效果。与单一IDR治疗组相比，Ad-PDCD5联合低剂量IDR治疗组肿瘤大小明显减小。Ad-PDCD5与低剂量IDR联合全身治疗、Ad-PDCD5局部注射与低剂量IDR内注射联合治疗的结果相似。这些结果表明，Ad-PDCD5可能是一种有前景的增强化学敏感性的药物。
PDCD5 (programmed cell death 5) accelerates apoptosis of certain tumor cells and is expressed at low levels in marrow-nucleated cells of AML and CML patients. In the present study, we evaluated the effects of PDCD5 overexpression on drug sensitivity of leukemia cells. K562 cells were treated with idarubicin (IDR) alone or in combination with adenoviral vectors expressing PDCD5 (Ad-PDCD5). As shown by annexin-V-FITC/PI dual labeling, apoptosis rates were markedly increased after combined treatment with Ad-PDCD5 compared to IDR treatment alone. We observed that PDCD5 overexpression significantly improves the antitumor effects of low dose IDR treatment in vivo. Tumor sizes were significantly decreased in combined Ad-PDCD5 and low dose IDR treatment groups compared with single IDR treatment groups. Similar results were obtained with combined systemic treatment of Ad-PDCD5 and low dose IDR, and combined treatment with Ad-PDCD5 local injection and low dose IDR i.p. injection. These results indicate that Ad-PDCD5 may be a promising agent for enhancing chemosensitivity.